In Situ Apoptosis Detection Kit

This kit is designed to detect fragmented DNA histochemically by terminal labeling. TUNEL method (Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) is an effective method for measuring the DNA fragments resulting from the apoptotic activation of intracellular endonucleases. Fluorescein labeled nucleotides are in situ incorporated onto the ends of these DNA fragments, allowing histologic localization and individual cells to be detected.

Features

• Quick and easy detection of apoptotic cells
• Sensitive detection of single cells in the initial stages of apoptosis
• Specific staining of apoptotic but not necrotic cells
• Flexible enough to be used with tissue section or fixed-cell samples
• Individual components are available separately

Applications

Fragmented-DNA detection by the TUNEL method
Detection of DNA strand breaks in apoptotic cells via fluorescence or light microscopy

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)