IgG Sepharose 6 Fast Flow affinity resin

lgG Sepharose 6 Fast Flow uses the rigid Sepharose 6 Fast Flow matrix, covalently coupled with human IgG, to purify protein A-containing proteins.

• Designed for rapid, single-step purification of protein A and protein A fusion conjugates.
• Binds at least 2 mg protein A/mL resin.
• Suitable for tandem affinity purification (TAP) of protein complexes.

Successful use in published research

Applications using IgG Sepharose 6 Fast Flow include:

• Tandem affinity purification (TAP) to obtain functional proteins from human cells
• Tandem affinity purification to study phosphorylation sites on the Rpb1 C-terminal domain of RNA Polymerase II
• Isolating protein A-Cvt19 to study the cytoplasm to vacuole targeting (Cvt) pathway

Compatible empty columns

Cytiva offers a wide range of Life Sciences solutions for protein purification, including several types of empty columns. lgG Sepharose 6 Fast Flow is compatible with gravity flow, Tricorn, HiScale, and XK column lines.

Convenient prepacked columns

IgG Sepharose Fast Flow resin is available in the following format:

• HiTrap IgG Sepharose FF: convenient for easy use with a syringe, peristaltic pump, or chromatography system

Documents

• Instructions for use (PDF)