Reagents for capture of human or humanized IgG antibodies as ligands in biomolecular interaction analyses. Designed for use with Biacore 8K+, and Biacore 8K (larger kit component volumes than in Human Antibody Capture Kit) but can be used with all other Biacore systems.
Binds an epitope in CH₂ domain of human IgG (all subclasses recognized).
Little or no cross-reactivity towards other human Ig isotypes.
No, or very low, cross-reactivity with IgG from other species, except other primates and rabbit.
Very stable binding – little or no dissociation of captured antibodies.
Intended Use
Human Antibody Capture Kit, type 2 is intended for capture of human or humanized IgG antibodies as ligands in biomolecular interaction analyses.
Anti-Human IgG (Fc) Antibody Description
Anti-Human IgG (Fc) antibody is a monoclonal mouse anti-human IgG (Fc) antibody of IgG1 subclass, prepared by affinity chromatography on Protein A. The antibody recognizes an epitope within the CH₂ domain in human IgG Fc of all subclasses. It is also known to bind to monkey (Cynomolgus) and rabbit IgG.
