HRV 3C Protease is a recombinant 3C protease derived from human Rhinovirus type 14 expressed in E. coli. This product is a highly purified 6xHN-tagged protein. The enzyme has the same activity as the native protein and cleaves a specific amino acid sequence (LeuGluValLeuPheGln ↓ GlyPro). It is useful for cleaving tag sequences from fusion proteins that contain a HRV 3C Protease cleavage site. Because this product has a N-terminal 6xHN tag (which is a modified His-tag), it can be easily eliminated from the protease reaction solution through immobilized metal affinity chromatography (IMAC) using TALON Metal Affinity Resin or His60 Ni Superflow Resin. Additionally, because the enzyme is active at 4℃, protein cleavage reactions can be performed under conditions that do not affect the activity or stability of the target protein. This product includes a Cleavage Control Fusion Protein. The Control Protein has a N-terminal 6xHN tag. Digestion of the Control Protein with HRV 3C Protease results in a 52 kDa TF protein and a 24 kDa GST peptide. Successful cleavage of the Control Protein can be easily confirmed using SDS-PAGE.
Features
Active at 4°C, allowing the cleavage reaction to proceed under conditions that do not affect the activity or stability of the recombinant target protein
6xHN tag allows removal of the 3C protease from the reaction using IMAC
