HiTrap Protein G HP are prepacked columns for general-purpose preparative purification of monoclonal and polyclonal antibodies from most species including rat.
First choice Protein G resin for routine purification of antibodies: ensures minimized sample dilution and high resolution.
The small bead size (34 μm) ensures narrow elution peaks containing concentrated material.
High protein recovery due to high binding capacity of 25 mg IgG/mL resin.
Broader selectivity compared with protein A ligand.
Convenient HiTrap format for easy connection to a syringe, peristaltic pump, or chromatography system such as ÄKTA system.
Protein G column for general-purpose antibody purification
HiTrap Protein G HP columns are prepacked with Protein G Sepharose High Performance resin. This protein G affinity resin is a good first choice for general-purpose capture of antibodies at laboratory scale, because it binds to a broader range of IgG from eukaryotic species and also binds to more subclasses of IgG than protein A-based resins do.
Use these columns for IgG purification from cow, sheep, horse, rat, mouse, and human sources, including ascites, serum, and cell culture supernatants.
HiTrap Protein G HP columns deliver high-performance, high-resolution purifications with a binding capacity of 25 mg IgG/mL resin and fast kinetics. Columns are stable over a wide pH range – operational pH 3 to 9, and pH 2 to 10 for cleaning.
Successful use in published research
Protein G HiTrap columns are relied upon for a variety of research applications, including:
Purification of primary antibody from rabbit for research on human lung cells
