Guide-it™ sgRNA Screening Kit

The Guide-it sgRNA Screening Kit contains PCR reagents to amplify genomic DNA from your target cells and recombinant Cas9 for the in vitro screening of your sgRNA to help you pick the best guide sequences before moving to experiments in living cells or animals. It is combined with the Guide-it In VItro Transcription kit as part of the Guide-it Complete sgRNA Screening System.

Features

• Four kits for simple production, cleanup, and evaluation of the efficacy of sgRNA (single guide RNA) for Cas9-mediated gene editing studies
• In vitro transcribe and purify high yields of sgRNA with the Guide-it sgRNA In Vitro Transcription Kit (Cat. # 632635; includes the Guide-it IVT RNA Clean-Up Kit, Cat. # 632638) for transfection or in vitro screening
• Use the Guide-it IVT RNA Clean-Up Kit (Cat. # 632638) to quickly purify sgRNA after IVT reactions, for direct use in transfection, electroporation, or in vitro cleavage assays
• High-quality recombinant Cas9 protein included in the Guide-it sgRNA Screening Kit (Cat. # 632639)

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)
• Guide-it sgRNA In Vitro Transcription Kit Components List
• Components

Kit Components

the necessary reagents for the transcription and cleanup of your sgRNA sequences