The FailSafe PCR System is the post optimization, bundled, and affordable kit that contains everything you need for successful PCR performance. The kit is multiplex PCR compatible, can amplify even the most difficult high-GC templates, and targets up to 20 kb in length.
The PCR system can be purchased after PCR conditions are optimised with the FailSafe PCR PreMix Selection kit, so you know which 2x Premix to choose for your template/primer combination.
Choose between 100, 250, or 1000 Enzyme Mix units, then combine with your top choice of 1, 2, or 8 out of the 12 PCR 2x PreMixes. The Enzyme Mix included has a fidelity of at least three times higher than Taq DNA polymerase.
The FailSafe 2x PreMixes and Enzyme Mix can be purchased with the PCR PreMix Selection Kit for initial optimization, with the PCR System, or, as individual components.
Applications
End point PCR applications
Cloning
Sequencing
Gene Expression
Mutation Analysis
How It Works
Step 1
Start with the FailSafe PCR PreMix Selection Kit to optimise end-point PCR conditions.
Step 2
Analyse and determine which FailSafe PCR 2x PreMixes produce high fidelity results, based on the template/primer pair combination.
Step 3
Purchase the post optimization FailSafe PCR System, which includes the Enzyme Mix plus your choice of 1, 2, or 8 out of the 12 PCR 2x PreMixes, OR, purchase the FailSafe PreMix and Enzyme components individually; and achieve reliable and consistent high-fidelity PCR results.
For a complete explanation of how FailSafe technology works, please read the manual.
Figure 1. Amplification of an 80%-85% GC-rich region of the human fragile X gene. PCR was performed using the FailSafe PCR System, schematically depicted above. Lanes A-L show the amplification products resulting from PCR using the 12 FailSafe PCR PreMixes. Lane M, molecular weight marker. In this experiment, optimal amplification was obtained with FailSafe PCR PreMix J. The size of the expected amplicon is indicated by an arrow. Figure 2. Multiplex PCR of the human CFTR gene. The FailSafe PCR System amplified all five exons of the CFTR gene from as little as 1 ng of human genomic DNA.
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Key features
- Bundled and affordable kit with the option to pick and choose your optimal FailSafe 2X PreMixes
- Consistent and high-fidelity PCR results
- Extremely high sensitivity and specificity
- Fidelity at least 3x higher than Taq DNA polymerase
Product Details
Before purchasing, we strongly recommend first optimising your PCR conditions using the FailSafe PCR PreMix Selection Kit in order to know which 2X PreMixes to choose with your PCR System kit.
Includes FailSafe PCR Enzyme Mix, a blend of thermostable enzymes containing a 3′→5′ proofreading enzyme for high fidelity, plus your choice of 1, 2, or 8 of the FailSafe PCR 2X PreMixes (100 uL each). 2X PreMixes contain dNTPs, buffer, different concentrations of MgCl2, and PCR Enhancer with betaine.
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