This is the standalone Enzyme Mix component of the FailSafe PCR PreMix Selection Kit for optimization, and the post optimisation FailSafe PCR System.
The Enzyme Mix is a unique blend of thermostable DNA polymerases, containing a 3′→5′ proofreading enzyme which helps achieve fidelity at least 3 times higher than Taq DNA polymerase. The thermostable enzymes may help against DNA denaturation when exposed to high temperatures, and may have a tolerance against certain inhibitors, making them ideal enzymes for every PCR need.1
Simply add your template, primers, and the FailSafe PCR Enzyme Mix to each of the FailSafe 2X PreMixes and amplify.
For a complete explanation of how FailSafe technology works, please read the manual.
Reference
1. Killelea T, Ralec C, Bossé A, Henneke G. PCR performance of a thermostable heterodimeric archaeal DNA polymerase. Front Microbiol. 2014 May 7;5:195. doi: 10.3389/fmicb.2014.00195. PMID: 24847315; PMCID: PMC4019886.
Key features
- Unique blend of thermostable enzymes
- 3′→5′ proofreading enzyme
- High fidelity at least 3 times higher than Taq DNA polymerase
Product Details
The PCR Enzyme Mix is a blend of thermostable DNA polymerases containing a 3′→5′ proofreading enzyme for high fidelity. Supplied in a 50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 0.5% Tween®20, 0.5% NP40, and 1 mM dithiothreitol.
Choose from 2 different sizes of FailSafe PCR Enzyme Mix, 100 Units (40 uL) and 1000 Units (400 uL).
Before purchasing we strongly recommend first optimising your PCR conditions using the FailSafe PCR PreMix Selection Kit. If optimization is complete, you can proceed with the bundled and affordable post optimisation FailSafe PCR System.
The “failsafe” nature is guaranteed when using all of the FailSafe components.
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