E. coli Exonuclease I is a 3'→5' exonuclease specific for single-stranded DNA, which results in the production of 5'-phosphate mononucleotides. This enzyme has a strict specificity for single-stranded DNA and does not react with double-stranded DNA or RNA. It is inactivated by heat treatment at 80°C for 15 minutes. Cat. # 2650B contains 5 of Cat. # 2650A. Please refer to Cat. # 2650A for complete product documentation and resources.
Features
Single-stranded DNA degradation
Removal of ssDNA fragments in a reaction mixture
PCR primer digestion post-reaction
Applications
Single-stranded DNA degradation Removal of ssDNA fragments in a reaction mixture PCR primer digestion post-reaction