E.Z.N.A.® Plasmid DNA Midi Kit

SKU: D6904-00

111.00 NIS

Stock on Request

In stock

Speed – Purification of plasmid DNA in < 60 minutes
Safety – No phenol/chloroform extractions
Versatile – Spin and vacuum formats available
High quality – DNA is suitable for a variety of downstream applications

The E.Z.N.A. Plasmid Midi and Maxi Kits can isolate up to 250 μg and 1.2 mg of plasmid DNA from 50 mL and 200 mL bacterial cultures, respectively. These kits use a modified alkaline lysis method to lyse the cells and separate genomic DNA from plasmid DNA. Cellular debris are removed by centrifugation, and the protocol follows a simple bind, wash, and elute procedure to deliver high-quality

plasmid DNA. The system not only eliminates the time-consuming isopropanol precipitation step required by gravity flow columns but also the need for expensive accessories. Purified plasmid DNA is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, ligation, PCR, in-vitro transcription, transformation, and other applications.

Specifications

Downstream Application	Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.
Starting material	15-50 mL LB culture; Optimal Cell Mass (OD600 x mL culture) is 80-100.
Plasmid type	High-copy, low-copy, cosmid DNA
Processing mode	Manual (centrifugation or vacuum)
Throughput	1 – 24
DNA binding technology	Silica Midi Spin Column
Lysate clearance method	Centrifugation
Processing time	<60 minutes
Yield	100-250 µg for high copy-number; 10-50 µg for low copy-number

Documents

Specifications

Binding technology Silica Midi Spin Column
Downstream application Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.
Plasmid type High-copy, low-copy, cosmid DNA
Processing mode Manual (centrifugation or vacuum)
Processing time <60 minutes
Starting material 15-50 mL LB culture; Optimal Cell Mass (OD600 x mL culture) is 80-100.
Sub category Bacterial Plasmid
Throughput 1 – 24
Yield 100-250 µg for high copy-number; 10-50 µg for low copy-number

links and documants

Protocol manual Click here
Quick guide Click here
Safety data sheet Click here
Source url Click here