E.Z.N.A.® Micro RNA Kit
Vendor: Omega Bio-tek
SKU: R7034-01
In stock
The E.Z.N.A.® Micro RNA Kit combines the reversible binding properties of the HiBind® matrix, a new silica-based material, with the highly efficient lysis ability of RNA-Solv® to extract micro and large (>200 nt) RNA from a wide variety of starting materials. Micro and large RNA are separated to allow for quantification into downstream applications.
The system uses RNA-Solv® to efficiently lyse cells and protect RNA from degradation. Following a chloroform separation step, RNA is applied to a HiBind® RNA column where large RNA binds. The flow-through is retained and applied to a HiBind® Micro RNA column to bind small RNA.
Specifications
| Downstream Application | RTPCR*, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation |
|---|---|
| Elution Volume | 15-30 μL for miRNA; 40-70 μL for >200 nt |
| Starting Material | Cultured eukaryotic cells or bacteria, or from animal,plant, or fungal tissues |
| Starting Amount | Up to 100 mg |
| RNA Yield | Up to 50 µg |
| Processing Mode | Spin column |
| Throughput | 1 – 24 |
| RNA Binding Technology | Silica technology |
| Binding Capacity | 100 µg for each |
Documents
Specifications
- Binding capacity 100 µg for each
- Binding technology Silica technology
- Downstream application RTPCR*, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation
- Elution volume 15-30 μL for miRNA; 40-70 μL for >200 nt
- Processing mode Spin column
- Starting amount Up to 100 mg
- Starting material Cultured eukaryotic cells or bacteria, or from animal,plant, or fungal tissues
- Sub category miRNA Isolation
- Throughput 1 – 24
- Yield Up to 50 µg
links and documants
- Safety data sheet Click here
- Source url Click here
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E.Z.N.A.® Micro RNA Kit
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