E.Z.N.A.® HP Total RNA Kit
Vendor: Omega Bio-tek
SKU: R6812-00
In stock
- Rapid – RNA isolation in 25 minutes
- Versatile – Spin and vacuum formats available
- Specialized System – Sample Homogenization and gDNA elimination in one step
- Safe – No phenol/chloroform extractions
- High-quality – RNA is suitable for a variety of downstream applications
The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from cultured cells or tissues. The kit includes RNA Homogenizer columns that integrate sample homogenization and genomic DNA elimination into one single step. The RNA purification process is simplified with HiBind® Mini Column technology and can be accomplished in 25 minutes. This convenient spin-column format requires no phenol or chloroform, avoids time-consuming steps such as CsCl gradient ultracentrifugation, precipitation with isopropanol or LiCl and allows for multiple samples to be processed in parallel. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, next-generation sequencing, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Specifications
| Downstream Application | RT-PCR, RT-qPCR, Northern blotting, next-generation sequencing, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation. |
|---|---|
| Starting Material | Cultured cells and tissues |
| Starting Amount | 1 x 107 eukaryotic cells or 25-30 mg tissue |
| Elution Volume | 40-70 μL |
| RNA Binding Technology | Silica Mini spin column |
| Processing Mode | Manual (centrifugation or vacuum) |
| Throughput | 1 – 24 |
| Final Purified Product | Total RNA (> 200 nt) |
| RNA Yield | Up to 100 µg |
| Processing Time | 25 min |
| Special Notes | RNA Homogenizer Column that integrates sample homogenization and gDNA elimination into a single step |
Documents
Specifications
- Binding technology Silica Mini spin column
- Downstream application RT-PCR, RT-qPCR, Northern blotting, next-generation sequencing, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
- Elution volume 40-70 μL
- Processing mode Manual (centrifugation or vacuum)
- Processing time 25 min
- Starting amount 1 x 107 eukaryotic cells or 25-30 mg tissue
- Starting material Cultured cells and tissues
- Sub category Cultured Cells and Tissue
- Throughput 1 – 24
- Yield Up to 100 µg
links and documants
- Protocol manual Click here
- Quick guide Click here
- Safety data sheet Click here
- Source url Click here
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E.Z.N.A.® HP Total RNA Kit
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