E.Z.N.A.® Gel Extraction Kit (V-spin)
Vendor: Omega Bio-tek
SKU: D2500-00
In stock
- Rapid – DNA recovery from an agarose gel in 15 min
- Specialized buffer system – Visual determination of optimum DNA binding for higher yields
- Safe – No phenol/chloroform extractions
- Versatile – Spin and vacuum formats available
- High-quality – DNA is suitable for a variety of downstream applications
The E.Z.N.A. Gel Extraction Kit uses spin-column technology to purify DNA fragments ranging from 100 bp to 10 kb from all grades of agarose gels with high recovery (> 80%). The kit uses a specialized binding buffer system that not only dissolves the gel slice and binds to the spin column but also includes a pH indicator for a visual representation of optimal pH for DNA binding. The bind step is followed by three rapid wash steps, and DNA is eluted with deionized water or elution buffer. Purified DNA is ready for a variety of downstream applications such as ligations, PCR amplification, restriction enzyme digestion, cloning, and various labeling reactions.
Specifications
| Downstream application | Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting |
|---|---|
| Elution volume | 30-50 µL |
| Starting material | Agarose gel slice |
| Starting amount | Up to 25 µg DNA |
| DNA recovered | >85% recovery, 70 bp to 20 kb |
| Processing mode | Manual, centrifugation/vacuum |
| Throughput | 1-12 |
| DNA binding technology | Silica mini spin column |
| Binding capacity | 25 μg |
| Processing time | <10 minutes |
Documents
Specifications
- Binding capacity 25 μg
- Binding technology Silica mini spin column
- Downstream application Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting
- Elution volume 30-50 µL
- Processing mode Manual, centrifugation/vacuum
- Processing time <10 minutes
- Starting amount Up to 25 µg DNA
- Starting material Agarose gel slice
- Sub category Gel Extraction
- Throughput 1-12
- Yield >85% recovery, 70 bp to 20 kb
links and documants
- Protocol manual Click here
- Quick guide Click here
- Safety data sheet Click here
- Source url Click here
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E.Z.N.A.® Gel Extraction Kit (V-spin)
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