E.Z.N.A.® Cycle Pure Kit (V-spin)
Vendor: Omega Bio-tek
SKU: D6492-00
In stock
- Rapid – Purification of PCR products in less than 10 minutes
- Safe – No Phenol/chloroform extractions
- Versatile – Spin and vacuum formats available
- High-quality – DNA is suitable for a variety of downstream applications
The E.Z.N.A.® Cycle Pure Kit is a convenient system for the fast and reliable purification of PCR products. The E.Z.N.A.® Cycle Pure Kit uses HiBind® technology to recover DNA bands from 100 bp to 10 kb free of oligonucleotides, nucleotides, and polymerase with yields exceeding 80%. The binding conditions of the HiBind® DNA Mini Columns are adjusted by the addition of a specially formulated buffer before adding the sample. Following a rapid wash step, DNA is eluted with deionized water or a low salt buffer. Purified DNA can be directly used for most downstream applications include T-A ligations, PCR sequencing, restriction enzyme digestion, or various labeling reactions.
Specifications
| Downstream application | T-A ligations, sequencing, restriction enzyme digestion, and various other labeling reactions |
|---|---|
| Elution volume | 30-50 µL |
| Starting material | ssDNA, dsDNA, PCR products |
| Starting amount | 5-50 µL |
| DNA recovered | >80% recovery, 100 bp to 10 kb |
| Processing mode | Manual (centrifugation or vacuum) |
| Throughput | 1-24 |
| DNA binding technology | Silica mini spin column |
| Processing time | <10 minutes |
| Special Notes | For clean-up of DNA fragments < 200 bp, please follow the protocol modification outlined in the product manual |
Documents
Specifications
- Binding technology Silica mini spin column
- Downstream application T-A ligations, sequencing, restriction enzyme digestion, and various other labeling reactions
- Elution volume 30-50 µL
- Processing mode Manual (centrifugation or vacuum)
- Processing time <10 minutes
- Starting amount 5-50 µL
- Starting material ssDNA, dsDNA, PCR products
- Sub category PCR Cleanup
- Throughput 1-24
- Yield >80% recovery, 100 bp to 10 kb
links and documants
- Protocol manual Click here
- Quick guide Click here
- Safety data sheet Click here
- Source url Click here
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E.Z.N.A.® Cycle Pure Kit (V-spin)
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