E-Z 96™ Total RNA Kit

SKU: R1034-00

2,308.00 NIS

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In stock

Isolate total RNA from cultured cells or soft tissues using 96-well plate format.

The E-Z 96™ Total RNA Kit is designed for isolation of total cellular RNA from up to 5 x 10⁵ cultured cells or soft tissues. This kit can process single or multiple samples in less than 60 minutes. Utilizing HiBind® silica plate technology, the need for phenol/chloroform extractions, CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl are eliminated. Samples are lysed in a denaturing lysis buffer which inactivates RNases. Binding conditions are adjusted and the lysate is transferred to a 96-well HiBind® RNA Plate where the RNA is purified via three wash steps. High-quality RNA is eluted in RNase-free water. RNA purified using the E-Z 96™ Total RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, and other downstream applications.

Specifications

Downstream Application	PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution Volume	50-75 μL
Starting Material	Culture cells
Starting Amount	up to 5×105 cells
Processing Mode	Manual, centrifugation/vacuum
Throughput	96 samples per run
RNA Binding technology	Silica membrane 96-well

Documents

Specifications

Binding technology Silica membrane 96-well
Downstream application PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution volume 50-75 μL
Processing mode Manual, centrifugation/vacuum
Starting amount up to 5×105 cells
Starting material Culture cells
Sub category Cultured Cells and Tissue
Throughput 96 samples per run

links and documants

Protocol manual Click here
Safety data sheet Click here
Source url Click here