ChIP sequencing (ChIP-seq) combines chromatin immunoprecipitation (ChIP) with next-generation sequencing (NGS) to identify the location of protein binding to DNA. The DNA SMART ChIP-Seq Kit generates indexed ChIP-seq libraries suitable for NGS on Illumina sequencing platforms. Library complexity is preserved from as little as 500 pg of double-stranded or single-stranded input DNA. The kit utilizes Clontech's DNA SMART technology; sequencing libraries are generated without the need for enzymatic cleanup or adapter ligations. All of the necessary reagents for producing ChIP-seq libraries are provided in this kit, including primer sets that contain Illumina high-throughput indexes. There are three different versions of the kit depending on the number and specific Illumina indexes desired. For more information on the indexes supplied in each kit visit: www.takarabio.com.
Features
Fast (one hour) cross-link reversal and elution of ChIP DNA
Ligation-independent adapter addition for ChIP-seq library preparation
Flexible DNA inputs: 100 pg–10 ng of either ssDNA or dsDNA
Generate Illumina-ready sequencing libraries in around 4 hours
Applications
ChIP Elute Kit: DNA elution and cross-link reversal for qPCR or ChIP-seq applications DNA SMART ChIP-Seq Kit: Sequencing library preparation for ChIP-seq from low-input, fragmented DNA Illumina-specific NGS sequencing library production
