DNA Polymerase I Large (Klenow) Fragment

DNA Polymerase I, Large (Klenow) Fragment is a DNA-dependent polymerase that lacks the 5′→3′ exonuclease activities of E. coli DNA Polymerase I from which it is derived. The 3′→5′ remains intact to remove 3’ overhangs, and along with the polymerase activity to fill in 5’ overhangs and create blunt end DNA fragments. The optimal incubation temperature of the enzyme is 25 °C and uses 10X DNA Polymerase Buffer A. Heat inactivation of the enzyme is 75 °C for 20 minutes.

Key features

• Forms blunt ends by removing 3’ overhangs or filling in 5’ overhangs
• Used in second-strand cDNA synthesis
• Generates DNA probes using random primers

Product Details

DNA Polymerase I Large (Klenow) Fragment includes Klenow DNA Polymerase at 5 U/µL and 10X DNA Polymerase Buffer A

Documents

• 10X DNA Polymerase Buffer A
• DNA Polymerase I Large (Klenow) Fragment
• DNA Polymerase I Large (Klenow) Fragment