DNA Polymerase I catalyzes the incorporation of nucleotides into double-stranded DNA in a 5'→3' direction. It also possesses a 3'→5' exonuclease (proofreading) activity. The 5'→3' exonuclease activity is useful for labeling DNA by nick translation. DNA Polymerase I is supplied in 50 mM potassium phosphate (pH 6.5), 1 mM DTT and 50% glycerol. Cat. # 2130B contains 5 of Cat. # 2130A. Please refer to Cat. # 2130A for complete product documentation and resources.
Features
DNA labeling by nick translation
DNA end blunting of 5'- and 3'-overhangs
cDNA synthesis from DNA or RNA template
Applications
DNA labeling by nick translation DNA end blunting of 5'- and 3'-overhangs cDNA synthesis from DNA or RNA template
Unit definition
One unit is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol of total nucleotides into acid-insoluble product in 30 minutes at 37°C and pH 7.4, using poly d(A-T) as the template-primer
