DNA Polymerase I (E.coli)

DNA Polymerase I catalyzes the incorporation of nucleotides into double-stranded DNA in a 5'→3' direction. It also possesses a 3'→5' exonuclease (proofreading) activity. The 5'→3' exonuclease activity is useful for labeling DNA by nick translation. DNA Polymerase I is supplied in 50 mM potassium phosphate (pH 6.5), 1 mM DTT and 50% glycerol.

Features

• DNA labeling by nick translation
• DNA end blunting of 5'- and 3'-overhangs
• cDNA synthesis from DNA or RNA template

Applications

DNA labeling by nick translation
DNA end blunting of 5'- and 3'-overhangs
cDNA synthesis from DNA or RNA template

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)