Takara's DNA Ligation Kit, Mighty Mix, is a one-solution premix ligation reagent that offers high efficiency ligations, particularly for blunt-ended ligation. The reaction can be performed for 5 minutes at 25°C (rapid protocol), or 30 minutes at 16°C (standard protocol) with equal results for both circular and linear DNA ligations. The 2X Ligation Mix allows small ligation reaction volumes (10 μl) when DNA is limiting. The kit includes sufficient reagent for 100 ligation reactions. Ligation products reaction can be used directly in bacterial transformation.
Features
Enables high-efficiency ligations for cohesive, blunt-end, and TA cloning
2X ligation mix adds convenience and eliminates extra pipetting and purification steps
Ligation reactions can be quickly performed at either 5 or 30 minutes depending on DNA type
Reaction volumes are scalable
Applications
Highly efficient and fast DNA ligation Small reaction volume ligation TA and blunt-end cloning
