Catalase (EC 1.11.1.6) is an ubiquitous antioxidant which catalyzes the conversion of hydrogen peroxide (H2O2) into water (H2O) and oxygen (O2). H2O2 is an oxidative stress inducer that can cause damage cells at relatively low concentrations. H2O2 is also precursor to certain oxidant radical species (e.g. hydroxyl radical). Catalase plays a crucial role to prevent oxidative damage to the cells.
Tribioscience catalase Assay Kit provides a rapid, simple, reproducible, and sensitive tool for catalase activity determination in plasma, serum, urine, and other samples. The catalase activity assay uses enzyme reactions to produce color and fluorescence with the probe. The color intensity of the reaction at 570 nm or fluorescence intensity at λex/em = 530/590 nm is directly proportional to catalase concentration in the sample. The fluorometric assay is more sensitive than colorimetric assay.
APPLICATIONS
Direct Assays: Catalase enzyme activity in serum, plasma, urine, and other bio-samples.
KEY FEATURES
Flexible: Suitable for colorimetric and fluorometric methods.
Accurate: Use 25 µL samples, detection ranges are 1.95-31.25 U/mL in 96-well plate the assay.
Simple and high-throughput: Two-steps procedure: just load-incubate-adding-incubate-read. Kits can be used as a robust method.
KIT CONTENTS
Component
Part Size
Assay Buffer
10 mL
Enzyme substrate (10x)
0.3 mL
Red Probe
0.12mL
H2O2 Standard (400 µM)
0.3 mL
Enzyme positive control
60 µL
Enzyme mix
0.12 mL
STORAGE AND HANDLING
Store kit at -20°C. Shelf life of 1year. Protect from light.
Shipment with blue ice. Avoid frozen and thaw circles.
TYPICAL DATA
The typical data is provided for only demonstration reference of standard curve shown in Fig.2 for fluorometric assay and Fig.3 for colorimetric assay.
