The Canine IL-1β Fast ELISA is a solid phase ELISA designed to measure canine IL-1β levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 2 hours, not 5-6 hours (Fig. 1). The detection range is from 7 to 500 pg/mL. The levels of canine IL-1β samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural canine IL-1β protein.
Typical Data
This standard curve (R2=1) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MDD) of canine IL-1β is typically 7 pg/mL.
The Intra-CV is < 10%, the Inter-CV is < 10%.
Specificity
This assay recognizes natural and recombinant canine IL-1β.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
PART
PART#
DESCRIPTION
STORAGE OF OPENED/ RECONSTITUTED
Canine IL-1β Microplate
TBS33001A
96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for Canine IL-1β.
The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.
Canine IL-1β Standard
TBS33001B
30 μL of Recombinant Canine IL-1β protein (25 ng/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS33001C
220 µL of Biotinylated Canine IL-1β antibody.
May be stored for up to 3 months at 2-8 °C.
Detection B
TBS33001D
300 µL of Streptavidin-HRP
Assay Diluent
TBS33001E
15 mL of a buffered protein base with preservatives.
10x Wash Buffer
TBS3000W
15 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
