An AmpliScribe T7-Flash Transcription reaction yields more RNA (180 µg per 20-µl reaction) than other high-yield in vitro transcription kit reactions. The AmpliScribe T7-Flash Kit utilizes proprietary enzyme formulations that enable the maximum possible yields of RNA from virtually any template that is downstream of the T7 RNA polymerase promoter, including sequences in linearized plasmids, double-stranded oligos or PCR products.
Features
Exceptionally high yields. Up to 180 µg of full-length RNA can be produced from 1 µg of 1-kb linearized plasmid DNA template. Scalable. Reactions can be scaled up to obtain grams (or more) of RNA. Fast. A standard AmpliScribe T7-Flash reaction is complete in 30 minutes. Convenient. Rnase inhibitor included. Trusted. Can be used for classic "Eberwine"-type RNA amplification procedures. read more
Key features
Generate maximum amounts of RNA - over 180 ug RNA from 1 ug DNA template
Save time with 30 minute high-yield protocol
Scale up the reaction to produce grams of RNA
Product Details
AmpliScribe T7-Flash Transcription Kits ASF3257(25 reactions) and ASF3507 (50 reactions) contain AmpliScribe T7-Flash Enzyme Solution, AmpliScribe T7-Flash 10X Reaction Buffer, ATP, CTP, GTP and UTP Solutions, RNase-Free Water, RNase-Free DNase I, DTT, Control DNA Template (linearized), and RiboGuard RNase Inhibitor. AmpliScribe T7-Flash Transcription Kit Item ID ASF250 (250 reactions) contains AmpliScribe T7-Flash Enzyme Solution and AmpliScribe T7-Flash 10X Reaction Buffer only.-
The "enzyme and 10X buffer only" format includes AmpliScribe T7-Flash Enzyme Solution andd 10X Reaction Buffer.
