Typhoon™ biomolecular imagers deliver exceptional data quality by utilizing highly sensitive detection, high image resolution, and a broad linear dynamic range. Typhoon™ laser scanners have been the researcher’s trusted choice for various applications for decades including:
Phosphor imaging for sensitive radioactive detection of DNA, RNA and other biomolecules.
Florescence imaging in RGB and NIR wavelengths for imaging of gels and blots- Northern, Southern, Western blotting, 2D DIGE, etc.
High resolution tissue section imaging using florescence or radioactive probes.
Special applications like microarrays, tissue section imaging, Gel-shift assays (EMSA) and scanning of multiwell plates that require high resolution, sensitivity & large scan area.
Ideal for core facilities, the Typhoon™ scanners are fully flexible in their configurations with four models to chose from. Typhoon™ laser scanners are easily upgradable allowing you to invest wisely in the features you need today whilst future-proofing your lab for tomorrow’s needs.
Compare Typhoon models by detection modes:
Amersham Typhoon IP
Amersham Typhoon NIR
Amersham Typhoon NIR Plus
Amersham Typhoon RGB
Amersham Typhoon 5
Phosphor imaging
✓
✓
✓
Densitometry (Optical density)
✓*
✓
✓
RGB fluorescence
✓**
✓
✓
Near-inrared fluorescence
✓
✓
✓
* Optical density accessory (OD plate) is needed
** Only Green fluorescence channel is included
Key Features
Accurate quantitation: detect signals from as low as 3 pg of protein and differences across a dynamic range with greater than five orders of magnitude
High resolution: resolve fine details in your sample with a pixel resolution of as low as 10 μm
High sample throughput: large scanning area of 40 × 46 cm enables the simultaneously imaging of up to 20 gels or blots, measuring 10 × 8 cm in size, or up to 9 multiwell plates in a single scan
Versatile: one system to image multifluorescent-,radioisotope-labeled, and colorimetric samples on gels, membranes, multiwell plates, culture dishes, glass slides, and tissue sections
Upgrade kits & custom filter box to offer the maximum options in fluorescence imaging and the best the Typhoon™ system has to offer
Despite advances in technology, scientists have been using phosphor imagers like the Typhoon™ laser scanner for radioactive detection because of its high sensitivity, resolution & large scan area (40x46 cm)
In genomics research phosphor imaging is routinely used along with techniques like NGS to detect trace amounts of radiolabeled DNA & RNA. Most common applications are RNA 5’ end modification, cleavage assays and fragment analysis
Quantitative whole body autoradiography (QWBA) is the method of choice to study the distribution of drug compounds in tissues with the Typhoon™ laser scanners being the gold standard in this highly regulated application
Storage phosphor screens BAS-IP, exclusively from Cytiva are quantitative & offer high sensitivity together with being easy to use & fast!
Image analysis software
For seamless data transfer and image analysis, there are two software options to use with the Typhoon™ imagers:
ImageQuant™ TL software, which facilitates gel and blot analysis, multiwell plate, array analysis, tissue sections and colony counting modules.
Melanie™ 2 software, which analyzes 2D gels for applications like2D-DIGE, and 2D-DIBE for HCP Coverage assay
A comprehensive suite of life cycle validation services is available for laboratory systems used in good practice environments, such as GLP, GMP, or GCP. The documentation is developed and approved by validation experts.
