High sensitivity chemiluminescence detection on Western blotting
Amersham ECL Western Blotting Detection Reagents use enhanced luminol-based detection, which is suitable for all routine Western blotting. ECL Western Blotting Detection Reagents, in combination with Amersham Protran membranes, form a complete solution for Western blot applications.
Reliable chemiluminescence detection
In most contemporary ECL systems a luminol peroxide detection reagent is added to the membrane and reacts with the horseradish peroxidase enzyme (HRP) conjugated to the secondary antibody. HRP catalyzes the oxidation of luminol in a multistep reaction and is accompanied by the emission of low-intensity light at 428 nm, which can be measured with light-sensitive X-ray film or with a CCD imager.
