Shrimp Alkaline Phosphatase (SAP) nonspecifically catalyzes the dephosphorylation of most phosphate monoesters, but it does not degrade diphosphate or triphosphate linkages. It is irreversibly inactivated by heat denaturation at 65°C for 15 minutes. Cat. # 2660B contains 5 of Cat. # 2660A. Please refer to Cat. # 2660A for complete product documentation and resources.
Features
Dephosphorylation of DNA 5'-termini
Reduction of vector re-ligation and background
Applications
Dephosphorylation of DNA 5'-termini Reduction of vector re-ligation and background