Bacterial Alkaline Phosphatase nonspecifically catalyzes the removal of most phosphomonoester bonds, but it does not degrade diphosphate or triphosphate linkages. The enzyme is purified from an E. coli strain lacks RNase. Alkaline Phosphatase is supplied in 50 mM Tris-HCl (pH 8.0), 100 mM KCl, 1 mM MgSO4 and 50% glycerol. Cat. # 2120B contains 5 of Cat. # 2120A. Please refer to Cat. # 2120A for complete product documentation and resources.
Features
Phosphate group removal to reduce self-ligation and vector background
Dephosphorylation of DNA 5' termini prior to kinase labeling reactions
Applications
Phosphate group removal to reduce self-ligation and vector background Dephosphorylation of DNA 5' termini prior to kinase labeling reactions
Unit definition
One unit is defined as the amount of enzyme required to generate 1 µmol/min of p-nitrophenol from p-nitrophenylphosphate at 25°C and pH 8.0
