Alkaline Phosphatase (E coli C75)

Bacterial Alkaline Phosphatase nonspecifically catalyzes the removal of most phosphomonoester bonds, but it does not degrade diphosphate or triphosphate linkages. The enzyme is purified from an E. coli strain lacks RNase. Alkaline Phosphatase is supplied in 50 mM Tris-HCl (pH 8.0), 100 mM KCl, 1 mM MgSO4 and 50% glycerol.

Features

• Phosphate group removal to reduce self-ligation and vector background
• Dephosphorylation of DNA 5' termini prior to kinase labeling reactions

Applications

Phosphate group removal to reduce self-ligation and vector background
Dephosphorylation of DNA 5' termini prior to kinase labeling reactions

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)