Calf Intestinal Alkaline Phosphatase (CIAP) catalyzes the hydrolysis of 5'-phosphate groups from DNA and RNA. CIAP is supplied in 10 mM Tris-HCl (pH 8.0), 50 mM KCl, 1 mM MgCl2, 0.1 mM ZnCl2 and 50% glycerol. Cat. # 2250B contains 5 of Cat. # 2250A. Please refer to Cat. # 2250A for complete product documentation and resources.
Features
Reduction of linear vector self-ligation and vector background during cloning
Dephosphorylation of DNA prior to kinase labeling reactions
Template preparation prior to 5'-end labeling
Applications
Reduction of linear vector self-ligation and vector background during cloning Dephosphorylation of DNA prior to kinase labeling reactions Template preparation prior to 5'-end labeling Protein dephosphorylation
