Advantage GC Genomic LA Polymerase Mix is a blend of TaKaRa Taq, a proofreading polymerase, and a hot-start antibody, supported by a buffer system optimized for long-range GC-rich PCR. Includes separate tubes of Advantage GC Genomic LA Polymerase mix and GC-melt buffer. dNTPs are not included and must be purchased separately.
Features
Designed for 'long and accurate' PCR amplification—amplify products as large as 48 kb
6.5X higher fidelity than wild-type Taq DNA polymerase—generates virtually error-free PCR products
High sensitivity—amplify regions of GC-rich DNA, such as a 1.9-kb region of the TGF-beta gene (GC content = 69%), from as little as 10 pg of template
Antibody-mediated hot start—increase specificity by preventing nonspecific amplification before thermal cycling
