The Accuris™ Smart-Q™ 100 & Smart-Q™ 800 Fluorometers are designed for the precise quantification of DNA, RNA, and protein samples. By utilizing well-established methodologies for fluorescence detection, the Smart-Q Fluorometers provide higher specificity and enhanced accuracy compared to the traditional method of UV-absorbance detection. Accurate results are particularly crucial in workflows involving:
Next Generation Sequencing
PCR
Transfection
Western blotting
Immunoassays
The Smart-Q Fluorometers are engineered to achieve optimal performance with Accuris’ Fluorescent DNA Quantification Kits and assay tubes; however, they also maintain compatibility with a range of kits offered by various other suppliers.
Intuitive Usability
Each instrument is equipped with a 7-inch color touchscreen, featuring an intuitive, icon-driven user interface. Preprogrammed protocols allow for quick & effortless assay set-up. Results are quickly calculated and displayed in less than 6 seconds, and saved to the instrument’s internal memory. All data can also be exported easily to a PC using a USB flash drive.
Accurate Quantification
The Smart-Q Fluorometers, along with their accompanying assay kits, are engineered for the precise measurement of fluorescent dye signals that are bound to targeted biological molecules. These specially formulated dyes exhibit selective affinity towards DNA, RNA, or proteins, and generate fluorescent signals exclusively upon binding to their respective targets. Both Smart-Q models include Blue and Red channels that allow for various fluorescent dyes to be used in nucleic acid and protein measurements.
The Smart-Q Fluorometers incorporate advanced curve-fitting algorithms to establish calibration curves from standard samples of known concentrations.
The concentration of an unknown sample, whether it be DNA, RNA, or protein, is determined by correlating its Relative Fluorescence Units (RFUs) with those of the standards employed during calibration. The measurement sensitivity for each type of assay is distinct and defined by its detection limits.
Options for Throughput
The Smart-Q portfolio includes two distinct models, each designed to meet specific laboratory requirements:
The Smart-Q 100 allows for single-sample processing in 0.5mL assay tubes (NS1020-T). This renders the Smart-Q 100 ideal for laboratories processing fewer samples.
The Smart-Q 800 caters to high-throughput workflows and allows for up to eight samples to be evaluated at once using individual 0.2mL tubes or 8 x 0.2mL strips tubes (Order no. F1001-T and F1001-T-8, ). The use of a standard 8-channel pipettor can streamline both the reagent preparation and sample transfer, making the Smart-Q 800 an efficient solution for workflows requiring parallel processing of multiple samples.
Accuris DNA Quantification Kits
Accuris DNA Quantification Kits are designed for accurate fluorescent quantification of dsDNA using an instrument such as the SmartDrop XF, Qubit™or Accuris™ Fluorometer. The reagents are highly selective for double-stranded DNA (dsDNA), and not for single-stranded DNA (ssDNA), RNA, protein, or nucleotides. Included with each kit are a concentrated assay reagent, dilution buffer, and ready-to-use DNA standards. The assay reagent is diluted using the buffer provided, sample is added (1 µL to 20 µL of sample is needed), and the concentration can be determined using an appropriate fluorometer instrument.
High Sensitivity dsDNA Assay Kit
The Accuris High Sensitivity kit provides an accurate method for quantitation of dsDNA samples when sensitivity is required. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.005 to 120 ng/µL, providing a detection range of 0.1−120 ng. 0.1 to 100 ng/ul, providing detection of 0.1 to 100 ng
Broad Range dsDNA Assay Kit
The Accuris Broad Range kit provides an accurate method for quantitation of dsDNA samples for a broad range quantity of DNA. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.2 to 2,000 ng/µL, providing a detection range of 4−2,000 ng.
