AAVpro® Extraction Solution

Isolation of AAV particles from AAV particle-producing cells is conventionally performed using the freeze-thaw or sonication methods. However, these methods are time consuming and require special equipment. The AAVpro Extraction Solution is a reagent for the extraction of AAV particles from AAV particle-producing cells. This reagent provides a simple and efficient method for AAV particle isolation; the reagents are added to virus-producing cells and viral particles are recovered by centrifugation. The extracted AAV particle solution contains only a small amount of host protein and nucleic acid contamination. The resulting viral extract is well-suited for cell infection or further purification of viral particles.

Features

• Three-fold increase in viral yield compared to freeze-thaw methods
• Simple method for AAV particle extraction from virus-producing cells
• Reduced host DNA and protein contamination
• Can be used for any AAV serotype

Applications

AAV particle extraction from virus-producing cells

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)