Archer VARIANTPlex Comprehensive Tissue and Blood Panel

Identify DNA profiles of multiple cancers with one panel

Efficiently detect single nucleotide variants, copy number variations, insertions and deletions in 460 genes along with microsatellite instability, tumor mutational burden, and homologous recombination deficiency status with targeted NGS relevant for colorectal, breast, melanoma, gastric, pancreatic, CNS, NSCLC, myeloid, lymphoid, and other pan-cancer research.

Key Benefits

• Customizable content — Combine pre-designed panels, create, and easily add targets to your existing assay with Assay Marketplace , or work directly with a design expert.
• Detect confidently — Anchored Multiplex PCR (AMP™) chemistry is designed for compatibility with a wide range of sample types, including low-input and potentially degraded samples such as FFPE tissue. Archer Analysis includes a unique outlier detection algorithm that leverages position-specific data to enable variant detection even at low allele frequencies, empowering you to detect confidently.
• Achieve efficiency — Streamlined workflows for your lab are enabled by your choice of reaction-sized lyophilized reagents or high-throughput liquid reagents, while parallel workflows across all Archer panels provide efficient genomic characterization.

Panel Specifications

*Input mass requirements vary depending on type and quality. Unless the tumor cellularity and sample quality are high, 50 ng of FFPE-derived nucleic acid should be considered the minimum recommendation. If input is not limiting, 200 ng is recommended.

Gene Targets

ABL1, ABL2, ACVR1, ACVR2A, AKT1, AKT2, AKT3, ALK, ANKRD26, APC, AR, ARAF, ARHGAP35, ARID1A, ARID1B, ARID2, ARID5B, ASXL1, ASXL2, ATM, ATR, ATRX, AURKA, AURKB, AXIN1, AXIN2, AXL, B2M, BAP1, BARD1, BCL2, BCL2L1, BCL2L11, BCL6, BCOR, BCORL1, BIRC3, BLM, BMPR1A, BRAF, BRCA1, BRCA2, BRIP1, BTG1, BTG2, BTK, CACNA1A, CALR, CARD11, CASP8, CBFB, CBL, CBLB, CBLC, CCND1, CCND2, CCND3, CCNE1, CD28, CD70, CD79A, CD79B, CD274, CDC25C, CDC73, CDH1, CDK4, CDK6, CDK8, CDK12, CDKN1A, CDKN1B, CDKN2A, CDKN2B, CDKN2C, CEBPA, CHD1, CHD3, CHD4, CHD8, CHEK1, CHEK2, CIC, COL5A1, CREBBP, CRKL, CSDE1, CSF1R, CSF3R, CTCF, CTLA4, CTNNA1, CTNNB1, CUL3, CUX1, CXCR4, CYSLTR2, DAXX, DCK, DDR2, DDX3X, DDX41, DHX15, DICER1, DMD, DNMT3A, DOT1L, EEF1A1, EEF2, EGFR, EGR3, EIF1AX, ELF3, EP300, EPAS1, EPCAM, EPHA2, EPHA3, EPHA7, EPHB1, ERBB2, ERBB3, ERBB4, ERCC1, ERCC2, ERCC4, ERG, ERRFI1, ESR1, ETNK1, ETV6, EWSR1, EZH2, FAM46C, FAM46D, FAM175A, FANCA, FANCC, FANCD2, FANCE, FANCF, FANCG, FANCI, FANCL, FAT1, FBXW7, FGF1, FGF2, FGF3, FGF4, FGF7, FGF8, FGF9, FGF19, FGFR1, FGFR2, FGFR3, FGFR4, FH, FLCN, FLNA, FLT1, FLT3, FLT4, FOXA1, FOXA2, FOXL2, FOXO1, FOXP1, FOXQ1, FUBP1, GATA1, GATA2, GATA3, GEN1, GLI1, GNA11, GNA13, GNAQ, GNAS, GPS2, GRIN2D, GRM3, H3F3A, H3F3B, H3F3C, HGF, HIST1H1C, HIST1H1E, HIST1H2BD, HIST1H3B, HIST1H3C, HNF1A, HOXB13, HRAS, HUWE1, ID3, IDH1, IDH2, IGF1R, IKBKE, IKZF1, IL6ST, IL7R, INPP4B, INPPL1, IRF2, IRF4, IRF6, IRS2, JAK1, JAK2, JAK3, JUN, KANSL1, KDM5A, KDM5C, KDM6A, KDR, KEAP1, KEL, KIF1A, KIT, KLF2, KLF4, KLF5, KMT2A, KMT2B, KMT2C, KMT2D, KRAS, KRT222, LAMP1, LATS1, LATS2, LEMD2, LRP1B, LUC7L2, LZTR1, MACF1, MAP2K1, MAP2K2, MAP2K4, MAP3K1, MAP3K4, MAP3K13, MAPK1, MAX, MCL1, MDM2, MDM4, MECOM, MED12, MEF2B, MEN1, MET, MGA, MGMT, MITF, MLH1, MLLT3, MPL, MRE11A, MSH2, MSH3, MSH6, MST1R, MTOR, MUC6, MUC16, MUTYH, MYC, MYCL, MYCN, MYD88, MYH9, NBN, NCOR1, NF1, NF2, NFE2L2, NFKBIA, NFKBIE, NIPBL, NKX2-1, NOTCH1, NOTCH2, NOTCH3, NOTCH4, NPM1, NRAS, NSD1, NTRK1, NTRK2, NTRK3, NUP93, PALB2, PARP1, PAX5, PAX8, PBRM1, PDCD1, PDCD1LG2, PDGFRA, PDGFRB, PGR, PHF6, PIK3C2B, PIK3C2G, PIK3CA, PIK3CB, PIK3CD, PIK3CG, PIK3R1, PIK3R2, PIK3R3, PIM1, PLCB4, PLCG1, PLCG2, PLXNB2, PMS1, PMS2, POLD1, POLE, POLQ, POLRMT, PPARG, PPM1D, PPP2R1A, PPP2R2A, PPP6C, PRDM1, PRKAR1A, PRKD1, PRKDC, PSIP1, PTCH1, PTEN, PTMA, PTPDC1, PTPN11, PTPRC, PTPRD, PTPRT, RAC1, RAD21, RAD50, RAD51, RAD51B, RAD51C, RAD51D, RAD52, RAD54L, RAF1, RARA, RASA1, RB1, RBBP6, RBM10, RECQL4, REL, RET, RHEB, RHOA, RICTOR, RIT1, RNF43, ROS1, RPL5, RPS6KB1, RPS14, RPTOR, RRAS2, RUNX1, RUNX1T1, RXRA, SAMD9, SAMD9L, SCAF4, SDHA, SDHB, SDHC, SDHD, SETBP1, SETD2, SF3B1, SH2B3, SH2D1A, SLC29A1, SLX4, SMAD2, SMAD3, SMAD4, SMARCA4, SMARCB1, SMC1A, SMC3, SMO, SOCS1, SOX2, SOX9, SOX17, SPEN, SPOP, SPTA1, SPTAN1, SRC, SRSF2, STAG1, STAG2, STAT3, STAT5B, STAT6, STK11, SUFU, SYK, TAF1, TBL1XR1, TBX3, TCEB1, TCF3, TCF7L2, TERT, TET1, TET2, TFRC, TGFBR1, TGFBR2, TGIF1, THRAP3, TLR4, TMSB4X, TNFAIP3, TNFRSF14, TP53, TP63, TRAF2, TRAF3, TRAF7, TSC1, TSC2, TSHR, TXNIP, U2AF1, U2AF2, UBA1, UNCX, USP9X, VHL, WT1, XPO1, XRCC2, XRCC3, ZBTB20, ZFHX3, ZMYM2, ZMYM3, ZNF750, ZRSR2

Documents

User guides and protocols

• VARIANTPlex-LAC Protocol for Illumina (PDF)
• VARIANTPlex-HT Protocol for Illumina (PDF)
• VARIANTPlex HS-HGC Protocol for Illumina (PDF)

Product inserts

• VARIANTPlex-HT Comprehensive Tissue & Blood Product Insert (PDF)
• VARIANTPlex Comprehensive Tissue & Blood Product Insert (PDF)

Posters

• Comprehensive genomic profiling using VARIANTPlex and FUSIONPlex panels along with Archer’s new tumor-only homologous recombination deficiency algorithm poster (PDF)

Ordering Information

HT kits are liquid reagents for high-throughput / automated workflows (Illumina only). For research use only. Not for use in diagnostic procedures.