Archer VARIANTPlex Complete Solid Tumor Panel

Identify DNA profiles of multiple cancers with one panel

Efficiently detect single nucleotide variants, copy number variations, insertions and deletions in 430 genes along with microsatellite instability, tumor mutational burden, and homologous recombination deficiency status with targeted NGS relevant for colorectal, breast, melanoma, gastric, pancreatic, CNS, NSCLC and other pan-cancer research.

The VARIANTPlex Complete Solid Tumor v2 panel is available with integrated HRD assessment. The VARIANTPlex Complete Solid Tumor v1 panel can be paired with the VARIANTPlex HRD Module to provide an HRD assessment on a by-sample basis.

Key Benefits

• Flexible, customizable insights — Combine pre-designed panels, create custom panel content, and easily add targets to your existing assay, while maintaining high performance with minimal workflow disruption.
• Detect confidently — Anchored Multiplex PCR (AMP™) chemistry is designed for compatibility with a wide range of sample types, including low-input and potentially degraded samples such as FFPE tissue. Archer Analysis includes a unique outlier detection algorithm that leverages position-specific data to enable variant detection even at low allele frequencies, empowering you to detect confidently.
• Achieve efficiency — Streamlined workflows for your lab are enabled by choice of reaction-sized lyophilized reagents or high-throughput liquid reagents, while parallel workflows across all Archer panels provide efficient genomic characterization.

Panel Specifications

*Input mass requirements vary depending on type and quality. Unless the tumor cellularity and sample quality are high, 50 ng of FFPE-derived nucleic acid should be considered the minimum recommendation. If input is not limiting, 200 ng is recommended.

Gene Targets

AKT1, BRAF, EGFR, ERBB2, FOXL2, GNA11, GNAQ, GNAS, HRAS, IDH1, IDH2, KIT, KRAS, MET, NRAS, PDGFRA, PIK3CA, RET, TERT, TP53, ALK, APC, AR, ATRX, CDK4, CDK6, CDKN2A, CTNNB1, DDR2, ERBB3, ERBB4, ESR1, FBXW7, FGFR1, FGFR2, FGFR3, H3F3A, HIST1H3B, JAK2, MAP2K1, MAP2K2, MTOR, MYC, NOTCH1, NOTCH2, NOTCH3, NOTCH4, NTRK1, NTRK2, NTRK3, POLD1, POLE, PTEN, RAF1, RB1, RICTOR, ROS1, SMAD4, SMO, VHL, ATM, BARD1, BRCA1, BRCA2, BRIP1, CDK12, CHEK1, CHEK2, FANCA, FANCL, PALB2, RAD51B, RAD51C, RAD51D, RAD54L, STK11, ABL1, ACVR1, AKT2, AKT3, ARID1A, ARID1B, ARID2, ATR, AURKA, B2M, BAP1, BCOR, BLM, BMPR1A, CCND1, CCND2, CCND3, CCNE1, CDH1, CDKN2B, CHD1, CIC, CSF1R, DAXX, DDX3X, DICER1, EIF1AX, EP300, EPCAM, ERCC1, ERCC2, EZH2, FANCI, FGF19, FGFR4, FH, FLCN, FLT1, FLT3, FLT4, FOXA1, FUBP1, H3F3B, HIST1H3C, HNF1A, JAK1, JAK3, KDM6A, KDR, KEAP1, KLF4, KMT2C, KMT2D, LZTR1, MAP3K1, MDM2, MDM4, MED12, MEN1, MLH1, MPL, MRE11A, MSH2, MSH3, MSH6, MUC16, MUTYH, MYCN, NBN, NF1, NF2, NKX2-1, NPM1, PBRM1, PIK3CB, PIK3R1, PLCB4, PMS2, PPP2R1A, PPP2R2A, PRKD1, PTCH1, PTPN11, RAD50, RAD51, RHOA, RNF43, SDHA, SDHB, SDHC, SDHD, SETD2, SF3B1, SMAD2, SMARCA4, SMARCB1, SRC, SRSF2, STAG2, SUFU, TGFBR2, TP63, TRAF7, TSC1, TSC2, TSHR, U2AF1, XRCC2, XRCC3, ABL2, ACVR2A, ARAF, ARHGAP35, ARID5B, ASXL1, ASXL2, AURKB, AXIN1, AXIN2, AXL, BCL2, BCL2L1, BCL2L11, BCL6, BCORL1, BIRC3, BTG1, BTG2, BTK, CACNA1A, CARD11, CASP8, CBFB, CBL, CD274, CD70, CD79A, CD79B, CDC73, CDK8, CDKN1A, CDKN1B, CDKN2C, CEBPA, CHD3, CHD4, CHD8, COL5A1, CREBBP, CRKL, CSDE1, CSF3R, CTCF, CTLA4, CTNNA1, CUL3, CYSLTR2, DMD, DNMT3A, DOT1L, EEF1A1, EEF2, EGR3, ELF3, EPAS1, EPHA2, EPHA3, EPHA7, EPHB1, ERCC4, ERG, ERRFI1, EWSR1, FAM175A, FAM46C, FAM46D, FANCC, FANCD2, FANCE, FANCF, FANCG, FAT1, FGF1, FGF2, FGF3, FGF4, FGF7, FGF8, FGF9, FLNA, FOXA2, FOXO1, FOXP1, FOXQ1, GATA1, GATA2, GATA3, GEN1, GLI1, GNA13, GPS2, GRIN2D, GRM3, H3F3C, HGF, HIST1H1C, HIST1H1E, HIST1H2BD, HOXB13, HUWE1, IGF1R, IKBKE, IKZF1, IL6ST, IL7R, INPP4B, INPPL1, IRF2, IRF4, IRF6, IRS2, JUN, KANSL1, KDM5A, KDM5C, KEL, KIF1A, KLF5, KMT2A, KMT2B, KRT222, LAMP1, LATS1, LATS2, LEMD2, LRP1B, MACF1, MAP2K4, MAP3K13, MAP3K4, MAPK1, MAX, MCL1, MECOM, MEF2B, MGA, MGMT, MITF, MLLT3, MST1R, MUC6, MYCL, MYD88, MYH9, NCOR1, NFE2L2, NFKBIA, NIPBL, NSD1, NUP93, PARP1, PAX5, PAX8, PDCD1, PDCD1LG2, PDGFRB, PGR, PHF6, PIK3C2B, PIK3C2G, PIK3CD, PIK3CG, PIK3R2, PIK3R3, PIM1, PLCG1, PLXNB2, PMS1, POLQ, POLRMT, PPARG, PPM1D, PPP6C, PRDM1, PRKAR1A, PRKDC, PSIP1, PTMA, PTPDC1, PTPRC, PTPRD, PTPRT, RAC1, RAD21, RAD52, RARA, RASA1, RBM10, RECQL4, REL, RHEB, RIT1, RPL5, RPS6KB1, RPTOR, RRAS2, RUNX1, RUNX1T1, RXRA, SCAF4, SETBP1, SH2D1A, SLX4, SMAD3, SMC1A, SMC3, SOCS1, SOX17, SOX2, SOX9, SPEN, SPOP, SPTA1, SPTAN1, STAG1, STAT3, SYK, TAF1, TBL1XR1, TBX3, TCEB1, TCF3, TCF7L2, TET1, TET2, TFRC, TGFBR1, TGIF1, THRAP3, TLR4, TMSB4X, TNFAIP3, TNFRSF14, TRAF3, TXNIP, UNCX, USP9X, WT1, XPO1, ZBTB20, ZFHX3, ZMYM2, ZMYM3, ZNF750

Documents

User guides and protocols

• VARIANTPlex-HT Protocol for Illumina (PDF)
• VARIANTPlex HS-HGC Protocol for Illumina (PDF)

Product inserts

• VARIANTPlex Complete Solid Tumor v2 Product Insert (PDF)
• VARIANTPlex-HT Complete Solid Tumor v2 Product Insert (PDF)
• VARIANTPlex-HT Complete Solid Tumor Panel Product Insert (PDF)
• VARIANTPlex Complete Solid Tumor Product Insert (PDF)

Posters

• Comprehensive genomic profiling using VARIANTPlex and FUSIONPlex panels along with Archer’s new tumor-only homologous recombination deficiency algorithm poster (PDF)

Flyers

• Flexible Customizable Comprehensive Genomic Profiling Solutions Flyer (PDF)
• Solid Tumor and Sarcoma Research Panels (PDF)

Ordering Information

HT kits are liquid reagents for high-throughput / automated workflows (Illumina only). For research use only. Not for use in diagnostic procedures.