Archer FUSIONPlex Sarcoma v2 Panel
Vendor: Archer
SKU: AB0133
In stock
Discover key sarcoma answers with a targeted RNA panel
Identify known and novel fusions, splice variants, single nucleotide variants, insertions, deletions, and relative expression with targeted NGS of 63 genes relevant for sarcoma research.
Detect more with Archer FUSIONPlex NGS panels for RNA.
Key Benefits
- Detect more — Comprehensive fusion identification is enabled by open-ended targeted amplification, delivering detection of both novel and known fusions, while Archer Analysis’s multifeatured pipeline provides data confidence and clear visualization of breakpoints.
- Less QNS — Anchored Multiplex PCR (AMP™) chemistry is designed for compatibility with a wide variety of sample types, including low-input and potentially degraded samples such as FFPE tissue.
- Achieve efficiency — Streamlined workflows for your lab are enabled by choice of reaction‑sized lyophilized reagents or high‑throughput liquid reagents, while parallel workflows across all Archer panels provide efficient genomic characterization.
- Customize content — Use Assay Marketplace to modify any panel to fit your lab’s needs or start from scratch to keep up with the pace of discovery.
Panel Specifications
| Targeted genes | 63 |
| Genomic alterations | Fusions, splicing, exon-skipping, SNVs, indels, expression |
| Input nucleic acid required* | ≥10 ng |
| Recommended number of reads | 2 M |
| Hands-on time | <3.5 hours |
| Total library prep time | 1.5 days |
| Platform compatibility | Illumina® and Ion Torrent™ |
| Reagent format | Lyophilized or liquid† |
| Supported sample types | FFPE, fresh frozen, cytology smear, FNA |
*Input mass requirements vary depending on type and quality. Unless the tumor cellularity and sample quality are high, 50 ng of FFPE-derived nucleic acid should be considered the minimum recommendation. If input is not limiting, 200 ng is recommended.
†Liquid reagents available for Illumina platform only.
Gene Targets
ALK, BCOR, BRAF, CAMTA1, CCNB3, CIC, CSF1, CTNNB1, EGFR, EPC1, ERG, ESR1, ETV1, ETV4, ETV5, ETV6, EWSR1, FGFR1, FGFR2, FGFR3, FOS, FOSB, FOXO1, FUS, GLI1, HMGA2, JAZF1, MBTD1, MDM2, MEAF6, MET, MGEA5, MKL2, MYOD1, NCOA1, NCOA2, NCOA3, NR4A3, NTRK1, NTRK2, NTRK3, NUTM1, PAX3, PDGFB, PDGFRA, PHF1, PLAG1, PRKCA, PRKCB, PRKCD, RAF1, RET, ROS1, SS18, STAT6, TAF15, TCF12, TFE3, TFG, USP6, VGLL2, YAP1, YWHAE
Documents
User guides and protocols
- FUSIONPlex Protocol for Ion Torrent (PDF)
- FUSIONPlex Protocol for Illumina (PDF)
- FUSIONPlex-HT Protocol for Illumina (PDF)
Product inserts
Ordering Information
| Cat. # | Kit | Format |
|---|---|---|
| AB0133 | FUSIONPlex Sarcoma v2 ILMN 8rxn | Illumina / 8 rxn |
| AB0134 | FUSIONPlex Sarcoma v2 IONT 8rxn | Ion Torrent / 8 rxn |
| AB0173 | FUSIONPlex-HT Sarcoma v2 ILMN 24rxn | HT / Illumina / 24 rxn |
| AB0174 | FUSIONPlex-HT Sarcoma v2 ILMN 96rxn | HT / Illumina / 96 rxn |
HT kits are liquid reagents for high-throughput / automated workflows (Illumina only). For research use only. Not for use in diagnostic procedures.
Specifications
- Assay time 1.5 days
- Content specifications 63 genes
- Hands on time <3.5 hours
- Input quantity ≥10 ng
- Platform compatibility Illumina and Ion Torrent
- Reagent type Lyophilized or liquid
- Specialized sample types FFPE, fresh frozen, cytology smear, FNA
- Sub category FUSIONPlex
- Variant class Fusions, splicing, exon-skipping, SNVs, indels, expression
links and documants
- Datasheet url Click here
- Protocol url Click here
- Source url Click here
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Archer FUSIONPlex Sarcoma v2 Panel
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