miniDAWN Multi-Angle Light Scattering Detector - Wyatt Technology

miniDAWN Multi-Angle Light Scattering Detector

Vendor: Wyatt Technology

SKU: WMD3-00

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DAWN

The Best in Basic Multi-Angle Light Scattering for Absolute Macromolecular Analysis.

Molar Mass

The miniDAWN determines absolute molar mass and size to characterize proteins, peptides, small polymers and nanoparticles in solution. Coupled to size exclusion chromatography (SEC or GPC), it eliminates the uncertainty and hassle of column calibration, for true confidence in macromolecular characterization.

Superb Sensitivity

The miniDAWN not only offers superb sensitivity—it further enhances productivity thanks to greatly enhanced field serviceability and easy data analysis with One-Click Mw^™.

Compatible with ASTRA^™

The miniDAWN is accompanied by ASTRA, the most versatile software package available for analysis of online multi-angle and dynamic light scattering. ASTRA and the miniDAWN incorporate advanced ease-of-use features that help guarantee excellent measurements with a single click.

Molar mass, absolutely

Wyatt's miniDAWN is most often used in conjunction with size exclusion chromatography (SEC-MALS) or field-flow fractionation (FFF-MALS) to determine distributions of molecular weight, size and composition. No need to make assumptions about molecular conformation or ideal column interactions.

Characterize:

Proteins, small peptides and oligonucleotides
Synthetic and natural polymers
Nanoparticles, virus-like particles and vesicles

Measurement Range:

MW from 200 Da to 10 MDa
r_g from 10 to 50 nm, up to 150 nm with shape-specific models
sensitivity down to 25 ng of 100 kDa polystyrene in THF, injected on a standard GPC column

protein molar mass characterization
with miniDAWN

The miniDAWN determines molecular weight independently of column calibration. Here MALS shows that the earliest-eluting molecule does not have the highest molar mass.

The miniDAWN's performance is exceeded only by the DAWN^™ which covers a wider range of size and molecular weight, with even better sensitivity and additional optional modules.

Ultrasonic Cleaning Included

Each miniDAWN includes an embedded COMET^™ ultrasonic transducer that cleans the flow cell automatically by agitating the fluid and loosening particles from the glass surfaces.

Modularity = Productivity

The miniDAWN incorporates a fully modular design. Each module can be tested independently by the software to verify that it is functioning correctly. This imparts nearly complete field serviceability, since malfunctioning modules can be replaced and validated on-site by service engineers with no further calibration or alignment.

The miniDAWN is also field-upgradeable: the embedded WyattQELS^™ DLS Module for measuring hydrodynamic radii from 0.5 to 50 nm can be installed on-site as well as in new units shipped from the factory.

Not just SEC-MALS...

Wyatt's miniDAWN may be used in batch (off-line) mode or in conjunction with a Calypso^™ CG-MALS system for characterization of average solution properties as well as macromolecular interaction properties including protein-protein binding affinity and complex stoichiometry.

Simplicity personified

With miniDAWN and ASTRA^™, SEC-MALS became a whole lot easier. Sample-specific methods are set up in three short steps with ASTRA's Method Wizard, and new capabilities for automatic data processing mean that it takes just one click from your sample to a full report of the molecular weight and size results.

The miniDAWN's System Ready monitor will even let you know when the system is fully equilibrated with low baseline noise, ready to run.

Specifications

Molar Mass Range	200 Da to 10 MDa (proteins) or 1 MDa (linear polymers)
Molecular Size Range ( R g )	≈ 10 nm to 50 nm, up to 150 nm with shape-specific models
Molecular Size Range ( R h )	In flow mode, 0.5 nm to 50 nm ; batch mode 0.5 nm to 1 μm (requires WyattQELS DLS module or DynaPro NanoStar + fiber optic connection)
Sensitivity	25 ng of 100 kDa polystyrene in THF or 500 ng of BSA in PBS, assuming standard 7.8 mm x 300 mm SEC column and no post-column filter
Mobile Phase Compatibility	All-solvent compatible (aqueous and organic); Wetted materials are 316 stainless steel, fused silica, and Kalrez
MALS Detectors	High-gain, high dynamic range photodiodes at 3 detection angles
Auxiliary Detectors	Laser monitor for stabilization feedback; forward transmission monitor to correct signals for absorbing samples and to assess data quality
Dynamic Range	3,300,000:1
DLS Detector (optional)	WyattQELS dynamic light scattering module installs directly inside the miniDAWN chassis. Alternatively, the optical fiber pickup of the DynaPro NanoStar cuvette-based DLS instrument or Mobius DLS-ELS instrument may be installed in the miniDAWN.
Laser Wavelength	658 nm ± 4 nm
Laser Power Control	Programmable 10% to 100%
Sample Temperature Control	Ambient
Analog Inputs	2 differential analog inputs with 24 bit resolution; Input range -10 V to +10 V
Analog Outputs	1 analog output from user selectable measurements channels -10 V to +10 V
Other Inputs/Outputs	Alarm in, Alarm out/retransmit, Auto-inject in, Auto inject contact closure retransmit, Recycle In/Out (operates Orbit recycle valve)
Computer Interface	Ethernet
Processed Data Rate	Up to 36.6 Hz
Front Panel Display	7" (178 mm), high-resolution, multi-touch display for viewing/setting signals, instrument settings and diagnostics
Dimensions	58 cm (L) x 36 cm (W) x 18 cm (H)

Documents

Brochure

Specifications

Dimensions 58 cm (L) x 36 cm (W) x 18 cm (H)
Instrument family miniDAWN
Laser wavelength 658 nm ± 4 nm
Mobile phase compatibility All-solvent compatible (aqueous and organic); Wetted materials are 316 stainless steel, fused silica, and Kalrez
Molar mass range 200 Da to 10 MDa (proteins) or 1 MDa (linear polymers)
Molecular size range Rg: ≈ 10 nm to 50 nm, up to 150 nm with shape-specific models; Rh: In flow mode, 0.5 nm to 50 nm ; batch mode 0.5 nm to 1 μm (requires WyattQELS DLS module or DynaPro NanoStar + fiber optic connection)
Sensitivity 25 ng of 100 kDa polystyrene in THF or 500 ng of BSA in PBS, assuming standard 7.8 mm x 300 mm SEC column and no post-column filter
Software ASTRA 8 (standard or with Security Pack)
Sub category Multi-Angle Light Scattering (MALS)

links and documants

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