Eclipse Field-Flow Fractionation System
Vendor: Wyatt Technology
SKU: WEC
In stock
The ultimate separation system for nanoparticles and macromolecules
Uniquely Versatile
Field-flow fractionation is a uniquely versatile means of size-based separations, capable of fractionating viral particles, liposomes and lipid nanoparticles, emulsions, proteins and aggregates, polysaccharides and technical polymers, engineered nanoparticles, colloidal soil suspensions, chemical mechanical polishing slurries, and many more - with no stationary phase and almost no shear. Separation of biotherapeutics can usually be done in full formulation buffer.
The Eclipse system is the foundation of FFF-MALS, a powerful technique for separation and comprehensive characterization of macromolecules and nanoparticles from 1 to 1000 nm in size. Eclipse brings FFF to a completely new level, combining exquisite performance and ease of use. Advanced features include the Dilution Control Module (DCM) and the ability to switch seamlessly between FFF and size-exclusion chromatography (SEC) separations. The Mobility add-on system expands Eclipse’s capabilities to include measurements of charge and zeta potential.
Simplicity and quality, with built-in intelligence
The Eclipse FFF system combines several modules that are integrated seamlessly in VISION™ software:
- The Eclipse FFF flow instrument
- Waters™ Arc™ HPLC pump and autosampler
- Industry standard pump and autosampler (legacy only)
- Eclipse FFF channel
These modules may be added:
- Industry standard fraction collector
- Mobility EAF4 system
Eclipse instrument
The Eclipse FFF instrument is the heart of the FFF system. It incorporates four highly precise and stable flow controllers that ensure robust and repeatable fractionation runs, without the associated pulsation and inherent complexity and low reliability of the multiple pumps found in other FFF systems.
Eclipse supports both tip injection and focus-zone injection, enabling use of a variety of FFF separation channels and even a size-exclusion chromatography column.
Optional features:
- FFF-SEC switching
- Dilution Control Module (DCM)
(see Options tab for more information)
The user interface is based on a 10” multi-touch display that lets users track progress of the method, know about events or conditions that need attention, and perform any necessary manual operations such as flushing the channel. Built-in intelligence provides health indicators and guided user assistance as well as self-diagnostics procedure.
Front end
The front end for an Eclipse FFF system comprises a Waters and other industry-standard pumps and autosampler. These components are recognized by analytical laboratories as state-of-the-art for reliability and performance:
- Low pump ripple and pulsation
- No wasted sample (no need to overfill the autosampler – 100 µL are loaded for a 100 µL injection)
- Used in tens of thousands of laboratories around the world, including in the demanding bio/pharmaceutical industry.
Eclipse FFF channels
Fractionation, which is what it’s all about, takes place in the open FFF channel which incorporates a semi-permeable (ultrafiltration) membrane supported by a frit. Eclipse channels offer several key advantages including extra-rigid construction, built-in temperature regulation and built-in dilution control ports. A variety of FFF channels are available to meet different needs – see the Channels tab to learn more.
VISION™
VISION software is the intelligent human interface to an FFF-MALS system built on Wyatt Technology’s Eclipse™ FFF and DAWN™ MALS instruments. It comprises two primary modules:
- VISION DESIGN™ for in silico method design and optimization
- VISION RUN™ for running FFF methods, with continuous system monitoring and self-diagnostics to ensure no wasted runs, lost sample or empty solvent bottle.
VISION interfaces with ASTRA™ software to coordinate synchronization and real-time display of light scattering data within VISION. Both software packages have been developed under a formal quality system and are available in 21 CFR Part 11 compliant options that fulfill GMP and GLP requirements.
Specifications
| Eclipse | Compatible with aqueous and organic solvents. Corrosion-resistant from pH 2 to 12. Wetted materials: 316 stainless steel, FFKM perfluoroelastomer, FR3 stainless steel, Vespel. Cross-flow regulation from 6.67 to 0.03 mL/min. Precision of detector flow rate: 0.1% (with DCM). Dedicated fraction collector output port for minimal peak broadening. |
| Communications | Ethernet |
| Inputs/Outputs | Alarm Out, Aux In with 18-bit effective resolution |
| Software | VISION software suite. Comprises VISION DESIGN module for in silico method development and optimization, and VISION RUN for running sequences and coordinating ASTRA data acquisition. |
| FFF-MALS Solution | Basic FFF-MALS system includes Eclipse instrument, one separation channel (matched to customer application), Waters and other industry-standard pump and autosampler, DAWN MALS instrument, VISION software suite and ASTRA light scattering analysis software. |
| Dilution Control Module | Factory-installed option. Increases sample concentration at the detector by up to a factor of 10 (up to 5 with negligible loss of resolution), controlled by software. |
| SEC Switching Option | Factory-installed option. Allows for automated software-controlled switching between SEC mode and FFF mode. |
| Mobility | Add-on option. Includes Mobility Module with pH sensor and conductivity sensor with software supported calibration, and Mobility channel. Maximum operating voltage ± 30 V, maximum current 30 mA; constant current or constant voltage mode. Wetted materials: platinum, titanium, polyetherimide, alumina ceramic, PTFE, Viton, PEEK, polycarbonate. |
| Detectors and Modules | PDA, UV/Vis, FLD, fraction collector, etc. |
| DLS | WyattQELS™ embedded dynamic light scattering module |
| Selection | Short, Long, Dispersion Inlet and Semi-Prep Channels available. Channel types are offered as fixed-height channel (without spacer) and variable-height channel (with spacer) options. All channels are compatible with aqueous and organic solvents and come with DCM port. Maximum pressure 30 bar. |
| Temperature Regulation | Ambient to 50 °C |
| Wetted Materials | 316 stainless steel, fused silica, FFKM perfluoroelastomer, alumina ceramic. Polycarbonate or Mylar according to the selected spacer for variable-height channels. |
| Spacers | Selection ranges from 250 μm to 1000 μm, depending on channel. FFKM perfluoroelastomer-coated polycarbonate for aqueous solvents, Mylar for organic solvents. |
| Membranes | Pre-cut polyethersulfone or regenerated cellulose with molecular-weight cutoff from 2 to 30 kDa. Templates available to cut other materials to size. |
| Dimensions | 58 cm (L) x 36 cm (W) x 26 cm (H) (Eclipse instrument) |
Documents
Specifications
- Dimensions 58 cm (L) x 36 cm (W) x 26 cm (H) (Eclipse instrument)
- Instrument family Eclipse
- Software VISION 4 (standard or with Security Pack)
- Sub category Field-Flow Fractionation
- Wetted materials 316 stainless steel, fused silica, FFKM perfluoroelastomer, alumina ceramic. Polycarbonate or Mylar according to the selected spacer for variable-height channels.
links and documants
- Brochure url Click here
- Source url Click here
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Eclipse Field-Flow Fractionation System
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