DAWN 8 Multi-Angle Light Scattering Detector - Wyatt Technology

DAWN Multi-Angle Light Scattering Detector

Vendor: Wyatt Technology

SKU: WD3-016500

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The world's most advanced light scattering instrument for absolute characterization of proteins, conjugates, macromolecules, and nanoparticles.

Premier MALS

The DAWN instrument is the premier Multi-Angle static Light Scattering (MALS) detector for absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution. It offers the highest sensitivity, the widest range of molecular weight, size, and concentration, and the largest selection of configurations and optional modules for enhanced capabilities.

Separation First

MALS is most often used in conjunction with a separation system such as Gel Permeation Chromatography (GPC-MALS), Size Exclusion Chromatography (SEC-MALS), or Field-Flow Fractionation (FFF-MALS) to determine distributions of mass, size, and composition, independent of column calibration by reference standards.

Supported by ASTRA^™ Software

ASTRA Software and its built-in analysis modules support the DAWN MALS instrument, providing the most versatile analysis available for online multi-angle and dynamic light scattering measurements.

Overview

The DAWN and its companion Optilab^™ dRI detector are the established benchmarks for MALS analysis, cited in thousands of peer-reviewed publications. Multi-angle light scattering detection is indispensable for use with GPC and HPLC-SEC in order to obtain reliable molecular mass distributions and information on molecular conformation, branching ratio, fragments and aggregates.

SEC-MALS with a DAWN and Optilab characterizes conformation and branching of synthetic and natural polymers larger than 10 nm in rms radius R_g. The addition of a ViscoStar^™ differential viscometer creates a GPC triple-detection system that can analyze molecular conformation and size below the R_g limit of 10 nm and determine Mark-Houwink Sakurada coefficients.

The combination of a UV/Vis absorbance detector with a DAWN instrument and Optilab refractive index detector creates a UV-MALS-RI triple-detection system, for analysis of copolymers and protein conjugates (glycoproteins, PEGylated proteins, surfactant-bound membrane proteins, etc.). With the addition of a WyattQELS^™ DLS module for size determination, protein conformation may be analyzed.

M_w from 200 Da to 1000 MDa
Sensitivity: 10 ng/mL 100 kDa polystyrene in THF, 0.4 µg/mL BSA (66.4 kDa) in PBS

R_g from 10 to 500 nm, up to 1000 nm with shape-specific models
Ultra-stable, high-power laser

The DAWN may also be used in batch (off-line) mode to characterize the weight-average molar mass, z-average size and second virial coefficient of unfractionated samples.

In conjunction with a Calypso^™ CG-MALS system, the DAWN characterizes macromolecular interactions, label-free and immobilization-free: affinity and absolute stoichiometry of self- and hetero-association of simple or complex molecular systems. The Calypso system also automates the determination of second virial coefficients, cross virial coefficients, average molar masses and sizes.

Ultrasonic Cleaning Included

Each DAWN instrument includes an embedded COMET^™ ultrasonic transducer that cleans the flow cell automatically by agitating the fluid and loosening particles from the glass surfaces.

Temperature-Controlled Models

Ambient model
Ultra-High-Temperature (UHT) model, from ambient up to +210 °C

Standard Peltier heated/cooled (H/C) model, temperature control from -15 °C to +150 °C

Specifications

Molar Mass Range	200 Da to 1 GDa
Molecular Size Range ( R g )	≈ 10 to 500 nm, up to 1000 nm with shape-specific models
Molecular Size Range ( R h )	In flow mode, 0.5 nm to 300 nm ; batch mode 0.5 nm to 1 μm (requires WyattQELS DLS module or DynaPro NanoStar + fiber optic connection).
Sensitivity	10 ng of 100 kDa polystyrene in THF or 200 ng of BSA in PBS, assuming standard 7.8 mm x 300 mm SEC column and no post-column filter
Mobile Phase Compatibility	All-solvent compatible (aqueous and organic). Wetted materials are 316 stainless steel, fused silica or F2 glass, and Kalrez.
MALS Detectors	High-gain, high dynamic range photodiodes at 18 detection angles.
Auxiliary Detectors	Laser monitor for stabilization feedback; forward transmission monitor to correct signals for absorbing samples and to assess data quality.
Dynamic Range	3,300,000:1
DLS Detector (optional)	WyattQELS dynamic light scattering module installs directly inside the DAWN chassis. Alternatively, the optical fiber pickup of the DynaPro NanoStar cuvette-based DLS instrument or Mobius DLS-ELS instrument may be installed in the DAWN.
Laser Wavelength	658 nm ± 4 nm; 785 nm optional for use with fluorescing samples
Laser Power Control	Programmable 10% to 100%
Flow Cells	Fused Silica, optimal for solvent refractive index less than 1.50; F2, optimal for solvent refractive index above 1.50
Temperature Options	Ambient Heated/Cooled (HC) -15 °C to +150 °C Ultra-High-Temperature (UHT) ambient to +210 °C
Analog Inputs	4 differential analog inputs with 24 bit resolution. Input range -10 V to +10 V
Analog Outputs	2 analog outputs from user selectable measurements channels -10 V to +10 V
Other Inputs/Outputs	Alarm in, Alarm out/retransmit, Auto-Inject in, Auto inject contact closure retransmit, Recycle In/Out (operates Orbit recycle valve)
Computer Interface	Ethernet
Front Panel Display	10.1" (257 mm), capacitive high-resolution screen with multi-touch functionality, displays signals, graphs, instrument settings and diagnostics
Dimensions	58 cm (L) x 36 cm (W) x 26 cm (H)

Documents

Brochure

Specifications

Applications Molar mass; Size; SEC-MALS; Conjugation; Conformation; FFF-MALS; DLS; Biomolecular interactions
Compatible with All-solvent compatible (aqueous and organic). Wetted materials are 316 stainless steel, fused silica or F2 glass, and Kalrez.
Dimensions 58 cm (L) x 36 cm (W) x 26 cm (H)
Instrument family DAWN
Key use Absolute molar mass and size of proteins, conjugates, polymers and nanoparticles
Laser wavelength 658 nm ± 4 nm; 785 nm optional for use with fluorescing samples
Mobile phase compatibility All-solvent compatible (aqueous and organic). Wetted materials are 316 stainless steel, fused silica or F2 glass, and Kalrez.
Molar mass range 200 Da to 1 GDa
Molecular size range Rg: ≈ 10 to 500 nm, up to 1000 nm with shape-specific models; Rh: In flow mode, 0.5 nm to 300 nm ; batch mode 0.5 nm to 1 μm (requires WyattQELS DLS module or DynaPro NanoStar + fiber optic connection).
Power requirements Programmable 10% to 100%
Sensitivity 10 ng of 100 kDa polystyrene in THF or 200 ng of BSA in PBS, assuming standard 7.8 mm x 300 mm SEC column and no post-column filter
Software ASTRA 8 (standard or with Security Pack)
Sub category Multi-Angle Light Scattering (MALS)
Technology 18-angle multi-angle light scattering (MALS) for SEC-MALS / FFF-MALS
Temperature range Ambient Heated/Cooled (HC) -15 °C to +150 °C Ultra-High-Temperature (UHT) ambient to +210 °C

links and documants

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