Calypso II Composition-Gradient System
Vendor: Wyatt Technology
SKU: WCG3
In stock
Label-free, immobilization-free characterization of protein-protein and other macromolecular interactions with composition-gradient multi-angle light scattering.
CG-MALS
The Calypso II, in conjunction with a DAWN™ or miniDAWN™ MALS detector, measures binding affinities and absolute, molecular stoichiometries of complex biomolecular interactions.
Weight-Average Molar Mass
In addition to equilibrium association constants Kd and absolute stoichiometry of reversible self- and hetero-associations, it can also help determine reaction or aggregation rates, non-specific interaction parameters (virial coefficients) and even automate measurements of weight-average molar mass and dn/dc for polymers.
CALYPSO™ Software
Included with each Calypso is a copy of CALYPSO software, the most versatile software package available for analysis of biomolecular interactions in solution by light scattering.
Creating composition gradients...
CG-MALS employs a Calypso connected to a MALS instrument and optional concentration detector. The Calypso performs sample preparation and delivery, combining up to three different solution in precise mixing ratios and injecting into the detectors.
Accurate compositions result from setting the Calypso's pumps to appropriate flow rate ratios, all controlled from the CALYPSO™ software through an intuitive GUI. Mixing is achieved by dispensing the solutions simultaneously from all three pumps via a static mixer.
...that work with MALS detectors
Reliable light scattering measurement require the samples to be degassed and filtered. The Calypso incorporates built-in, low-volume degassers and filter housings, connected via PEEK tubing and finger-tight fittings. Even the filter housing is finger-tight, making use of a proprietary design for maximum convenience.
Calypso features
- Biocompatible wetted materials.
- Flexibility to re-organize fluid paths and utilize different wetted materials as needed.
- Convenient loading of sample and diluent solutions via conical tubes: 5, 15 or 50 mL.
- Autoinject Out contact closure signal, to trigger data acquisition by ASTRA™ or DYNAMICS™ software for data acquisition and analyses not supported by the CALYPSO software (e.g., DLS acquisition).
- Built-in Wash port, allowing the pumps to draw on reservoirs of wash solutions for post-experimental clean-up. Automation of up to two sequential wash solutions, e.g. buffer (to clean out proteins) followed by water (to clean out buffer salts) or detergent followed by water, is possible when an Orbit™ solvent selector valve is added to the setup. These operations are appended to the Method for unattended operation.
Specifications
| Syringe Pumps | 3 computer-controlled pumps; dc-servo motor, for minimum pulsation; 3-port distribution valve on each pump for Input, Output and Wash |
| Syringe Volumes | 12.5 µL to 2.5 mL (1 mL and 0.5 mL syringes supplied as standard) |
| Degasser Channels | 1 per pump, 60 µL internal volume |
| Pressure Limit | 1 mL syringes: 200 psi 0.5 mL syringes: 400 psi |
| Auxiliary Inputs/Outputs | Autoinject out, injector valve control, recycle valve control, digital and analog I/O for specific applications |
| Solvents | Aqueous |
| Wetted Materials | Borosilicate glass, PEEK, PTFE, polyethylene, alumina, titanium, stainless steel |
| Typical Sample Volume Per Analysis | 2.5 to 7 mL, depending on detector configuration and complexity of analysis |
| Range of Measurement | Equilibrium Dissociation Constant, K D : 100 pM to 1 mM typical for 100 kDa molecules (actual range varies with molecular weight and association stoichiometry) |
| Repeatability of Measurement | M w : ± 5% A 2 : ± 0.5 x 10 mol·mL/g² for sample molar mass of 50 to 100 kDa log( K D ): ± 0.3 |
| Specific Binding | See CALYPSO Software for more details. Self-association Hetero-association Multi-valent Combined self- and hetero-association incompetent (inactive) fraction Cooperativity and competitive binding Binding with thermodynamic non-ideality (high concentration) Concatenate multiple data sets for global fit |
| Non-Specific Interactions | Self-interaction: 2 and 3 virial coefficients Hetero-interactions: cross virial coefficients with 2 self-virial coefficients |
| Kinetics | 1 -order analyzed in software; export data for higher-order kinetics |
| Additional analyses | dn/dc |
| Host PC Requirements | USB port for Calypso Ethernet connection for DAWN / miniDAWN communications |
| Dimensions | 58 cm (L) x 37 cm (W) x 35 cm (H) |
Documents
Specifications
- Dimensions 58 cm (L) x 37 cm (W) x 35 cm (H)
- Instrument family Calypso
- Software CALYPSO
- Sub category Composition-Gradient MALS
- Wetted materials Borosilicate glass, PEEK, PTFE, polyethylene, alumina, titanium, stainless steel
links and documants
- Brochure url Click here
- Source url Click here
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Calypso II Composition-Gradient System
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