Western BLoT Ultra Sensitive HRP Substrate allows detection of very small amounts of protein during Western blot analysis. This chemiluminescence substrate offers the highest sensitivity, and can be used to detect horseradish peroxidase (HRP)-labeled antibodies bound to target proteins on immunoblots. It is possible to detect protein levels in the femtogram range using this product. This substrate maintains a strong emission signal with linear signal across a wide dynamic range, allowing you to take full advantage of CCD camera detection sensitivity. Since background is very low, this system can be used for long exposure times, enabling detection of very small amounts of target protein. This product is useful when detecting minute amounts of target protein or when a limited amount of primary antibody is available. Western BLoT Ultra Sensitive HRP Substrate can be used for X-ray film detection. However, since the emission intensity is extremely high, it may be necessary to limit the exposure time and adjust the antibody dilution when using X-ray film.
