Western BLoT Rapid Detect v2.0 is a Western blot detection reagent kit used to detect primary antibodies using an HRP-labeled IgG Detector instead of a labeled secondary antibody. IgG Detector, the main component of this product, is a particle with approximately 100 protein molecules on its surface that bind to the Fc region of an antibody, and is labeled with about 50 molecules of HRP. This reagent enables quick, high-sensitivity detection and can be used for simultaneous detection of multiple primary antibodies. Moreover, the use of the included Dilution Buffer, which contains a blocking component, allows for higher sensitivity and simpler use. For the highest sensitivity and quickest detection for Western blotting, use this product in combination with the Western BLoT HRP Substrate series.
Features
Simultaneous detection of multiple primary antibodies
Detection of minute quantities of antigen that cannot be detected using conventional techniques with a labeled secondary antibody
Applications
Western blot signal detection without using a labeled secondary antibody Secondary antibody enhancement: IgG detector can be used after conventional techniques (labeled secondary antibody) to intensify the signal Re-probing blots that produced a weak signal with conventional techniques (labeled seco
