EmbryoMap Kit for PGT-A on the Illumina MiSeq

EmbryoMap is Vitrolife's NGS solution for labs assessing preimplantation embryo biopsy samples (PGT-A): it detects whole- and sub-chromosomal copy-number imbalances on the Illumina MiSeq. Each kit prepares 96 sequencing-ready libraries and comes with the matching MiSeq sequencing reagents under one reference number, in formats for 8, 12, 24 or 48 samples per run.

Kit Contents

The EmbryoMap Kit includes 1 x EmbryoMap Sample Prep (96 rxns), a single box containing reagents for the preparation of 96 sequencing-ready libraries and 2 x Illumina MiSeq Sequencing Reagents, MiSeq Reagent Kit v3 – Box 1 of 2 and MiSeq Reagent Kit v3 – Box 2 of 2 for sequencing on the Illumina MiSeq System.

The other formats differ only in the number of MiSeq sequencing reagent kits supplied with the 96-reaction sample prep box - see Ordering Information below.

Workflow

EmbryoMap for labs assessing preimplantation embryo biopsy samples

Through evolution and innovation, the EmbryoMap solution has been analytically validated for detection of whole- and sub-chromosomal imbalances with highly reproducible copy-number, using cell line reference material and embryo biopsy samples

Samples are prepared for sequencing using the EmbryoMap Sample Prep Kit, sequenced on the Illumina MiSeq Sequencing System and data analysis and reporting performed using eMap software

Streamlined Workflow

The EmbryoMap Sample Prep Kit and the Illumina MiSeq Sequencing Reagents can be conveniently ordered under a single reference number.

Kit formats are available for sequencing from 8 up to 48 samples per sequencing run.

Making shipping and storage convenient and recognising the need for sustainability, the EmbryoMap Sample Prep Kit is delivered in a single box with a small footprint. With the benefit of master-mixed reagents and pre-plated indexes, pipetting steps are minimised saving precious time.

Sequencing on the Illumina MiSeq

A custom sequencing recipe on the Illumina MiSeq System optimises output and automatically uploads run data to the eMap analysis software. Paired end reads are implemented for more reliable sequencing data alignment, while maintaining exceptional quality with more samples per run.

eMap Data Analysis

The cloud-based eMap software tool enables run planning, sample tracking, data analysis and reporting. The enhanced copy-number calling algorithm includes automated and manual calling options for full copy-number, sub-chromosomal and putative mosaic imbalances. Data can be visualised in a traditional CNV chart and new circos map. User-defined thresholds can be tailored to meet laboratory needs.

Reporting

The cloud-based eMap software allows users to generate reports, enabling customised features to be incorporated. Cycle and embryo reports can include logos, custom text, quality metrics and specifications for calling criteria.

Video

Documents

• SDS Extraction Mix (PDF)
• SDS Library Index Plate A (PDF)
• SDS Elution Buffer (PDF)
• SDS Amplification Mix 1 (PDF)
• SDS Amplification Mix 2 (PDF)

Ordering Information

This product is labeled for Research Use Only - Not for use in diagnostic procedures.