Vero Cell Host Cell Protein ELISA

The Tribioscience Vero cell HCP Fast ELISA is designed to quantitatively detect Vero cell HCP residues in different biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay flexible, accurate, simple, and time saving. The measurement can be finished in 2 hours, not needing 4-5 hours (Fig. 1). The detection range is from 1 to 200 ng/mL. The levels of Vero HCP samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for Vero host cell protein.

 

Typical Data

This standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

 

Sensitivity

LOD is about 1.1 ng/mL

LOQ is 4.9 ng/mL

The Intra-assay CV is 3.79% and the Inter-assay CV is ˂10%.

 

Specificity

This assay recognizes Vero HCP.

 

Simple Procedures

Kit Content and Storage Conditions

PART	PART#	DESCRIPTION	STORAGE OF OPENED/ RECONSTITUTED
Vero HCP Microplate	TBS31005A	96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific to Vero HCP.	Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Vero HCP Standard	TBS31005B	90 µL of Vero HCP protein (2 µg/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS31005C	55 µL of HRP Vero HCP antibody.	May be stored for up to 3 months at 2-8 °C.*
Assay Diluent	TBS31005D	25 mL of a buffered protein base with preservatives.
Wash Buffer	TBS3000W	15 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration date.

The kit contains sufficient materials to run an ELISA on one 96 well plate.

Research use only.