Urinary Pathogen ID/AMR Enrichment Kit Set B (RUO) (96 indexes, 96 samples)
Vendor: Illumina
SKU: 20090309
In stock
The Urinary Pathogen ID/AMR Enrichment Kit (UPIP) contains all library prep reagents, indexes and panel used to target 3,500 antimicrobial resistance (AMR) markers, 121 bacteria, 14 fungi, and 35 viruses and 4 parasites. The kit includes the Illumina DNA prep with Enrichment kit and analysis with the UPIP Explify app on BaseSpace Sequence Hub, the UPIP enrichment panel enables broad range detection of urinary pathogens and profiling of AMR to identify mixed co-infections. Kit contains library prep reagents, indexes (Set B) and panel to prepare 96 samples at 3-plex and analysis with the UPIP Explify app.
The Urinary Pathogen ID/AMR Panel (UPIP) is a next-generation sequencing (NGS) research panel that enables detection and quantification of over 170 uropathogens responsible for urinary tract infections (UTIs) and co-infections and covers more than 3700 markers and their alleles associated with antimicrobial resistance (AMR).
Key Features
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Delivers simultaneous detection of 120+ bacteria, 35 viruses, 14 fungi, 4 parasites, and 27 sexually transmitted pathogen plus associated AMR alleles in < 24 hours using the MiniSeq and Rapid Reagent Kit
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Enriches uropathogen target regions from a range of host and environmental samples
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Detects less common, challenging-to-grow, and frequently missed uropathogens
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Outperforms shotgun metagenomics and conventional urine culture methods
Streamlined workflow
The Urinary Pathogen ID/AMR Enrichment Panel Kit contains all library prep and enrichment reagents, indexes, and panel probes for on-bead tagmentation followed by a single hybridization step for generating enriched libraries. Integration of the enrichment kit with DNA extraction, sequencing, and DRAGEN Microbial Enrichment Plus allows a comprehensive and simple workflow for detection and identification of UTIs.
Specifications
| Assay time | < 9 hr library prep and enrichment time |
|---|---|
| Automation capability | Liquid handling robot(s) |
| Content specifications | Detects 120+ bacteria, 14 fungi, 35 viruses, 4 parasites, including 27 sexually transmitted pathogens, and > 3700 antimicrobial resistance markers |
| Description | A streamlined workflow that incorporates DNA extraction, library preparation using the Urinary Pathogen ID/AMR Enrichment Kit, sequencing, and DRAGEN Microbial Enrichment Plus Data Analysis for detection and identification of uropathogens and antimicrobial resistance markers from a range of sample types. |
| Hands-on time | < 2 hr library prep and enrichment time |
| Input quantity | Based on volume (not concentration dependent) |
| Instruments | MiSeq System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode, MiSeq i100 System, MiSeq i100 Plus System |
| Mechanism of action | On-bead tagmentation followed by a single hybridization step |
| Method | Targeted DNA sequencing, Target enrichment |
| Multiplexing | Up to 384 samples in a single run with unique dual indexes |
| Nucleic acid type | DNA |
| Sample type details | DNA extracted from human urinary clinical research samples and other surveillance related samples such as wastewater |
| Species category | Other, Human, Virus, Bacteria |
| Species details | fungi, parasites |
| Technology | Sequencing |
Required Products
No accessories needed.
Documents
- UPIP Full Feature Demo Report
- Precision Metagenomics Flyer
- UPIP ID Week Scientific poster
- Urinary Pathogen ID/AMR Panel flyer
- Illumina Urinary Pathogen ID/AMR Panel content
- Antimicrobial resistance surveillance using the Urinary Pathogen ID/AMR Enrichment Kit
- Wastewater surveillance using the Urinary Pathogen ID/AMR Panel
- Urinary Pathogen ID/AMR Panel Data Sheet
Support Documentation
Specifications
- Area of interest Microbiology
- Assay time < 9 hr library prep and enrichment time
- Automation capability Liquid handling robot(s)
- Content specifications Detects 120+ bacteria, 14 fungi, 35 viruses, 4 parasites, including 27 sexually transmitted pathogens, and > 3700 antimicrobial resistance markers
- Hands on time < 2 hr library prep and enrichment time
- Input quantity Based on volume (not concentration dependent)
- Instruments MiSeq SystemMiSeq i100 Plus SystemMiSeq i100 SystemMiSeqDx in Research ModeMiniSeq SystemNextSeq 1000 SystemNextSeq 2000 SystemNextSeq 550 SystemNextSeq 550Dx in Research Mode
- Key use Metagenomics, Targeted analysis, Whole-genome analysis
- Mechanism of action On-bead tagmentation followed by a single hybridization step
- Method Target enrichment, Targeted DNA sequencing
- Multiplexing Up to 384 samples in a single run with unique dual indexes
- Nucleic acid type DNA
- Species category Bacteria, Human, Other, Virus
- Sub category Library preparation kits
- Technology Sequencing
links and documants
- Datasheet url Click here
- Source url Click here
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Urinary Pathogen ID/AMR Enrichment Kit Set B (RUO) (96 indexes, 96 samples)
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