Urinary Pathogen ID/AMR Enrichment Kit Set A (RUO) (96 indexes, 96 samples)
Vendor: Illumina
SKU: 20090308
In stock
Includes all reagents necessary for library prep and enrichment including tagementation and PCR reagents, IDT for Illumina DNA/RNA UD Indexes Set A, and Urinary Pathogen ID/AMR Panel to prepare libraries from 96 samples followed by 32, 3-plex enrichment reactions.
The Urinary Pathogen ID/AMR Panel (UPIP) is a next-generation sequencing (NGS) research panel that enables detection and quantification of over 170 uropathogens responsible for urinary tract infections (UTIs) and co-infections and covers more than 3700 markers and their alleles associated with antimicrobial resistance (AMR).
Key Features
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Delivers simultaneous detection of 120+ bacteria, 35 viruses, 14 fungi, 4 parasites, and 27 sexually transmitted pathogen plus associated AMR alleles in < 24 hours using the MiniSeq and Rapid Reagent Kit
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Enriches uropathogen target regions from a range of host and environmental samples
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Detects less common, challenging-to-grow, and frequently missed uropathogens
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Outperforms shotgun metagenomics and conventional urine culture methods
Streamlined workflow
The Urinary Pathogen ID/AMR Enrichment Panel Kit contains all library prep and enrichment reagents, indexes, and panel probes for on-bead tagmentation followed by a single hybridization step for generating enriched libraries. Integration of the enrichment kit with DNA extraction, sequencing, and DRAGEN Microbial Enrichment Plus allows a comprehensive and simple workflow for detection and identification of UTIs.
Specifications
| Assay time | < 9 hr library prep and enrichment time |
|---|---|
| Automation capability | Liquid handling robot(s) |
| Content specifications | Detects 120+ bacteria, 14 fungi, 35 viruses, 4 parasites, including 27 sexually transmitted pathogens, and > 3700 antimicrobial resistance markers |
| Description | A streamlined workflow that incorporates DNA extraction, library preparation using the Urinary Pathogen ID/AMR Enrichment Kit, sequencing, and DRAGEN Microbial Enrichment Plus Data Analysis for detection and identification of uropathogens and antimicrobial resistance markers from a range of sample types. |
| Hands-on time | < 2 hr library prep and enrichment time |
| Input quantity | Based on volume (not concentration dependent) |
| Instruments | MiSeq System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode, MiSeq i100 System, MiSeq i100 Plus System |
| Mechanism of action | On-bead tagmentation followed by a single hybridization step |
| Method | Targeted DNA sequencing, Target enrichment |
| Multiplexing | Up to 384 samples in a single run with unique dual indexes |
| Nucleic acid type | DNA |
| Sample type details | DNA extracted from human urinary clinical research samples and other surveillance related samples such as wastewater |
| Species category | Other, Human, Virus, Bacteria |
| Species details | fungi, parasites |
| Technology | Sequencing |
Required Products
No accessories needed.
Documents
- UPIP Full Feature Demo Report
- Precision Metagenomics Flyer
- UPIP ID Week Scientific poster
- Urinary Pathogen ID/AMR Panel flyer
- Illumina Urinary Pathogen ID/AMR Panel content
- Antimicrobial resistance surveillance using the Urinary Pathogen ID/AMR Enrichment Kit
- Wastewater surveillance using the Urinary Pathogen ID/AMR Panel
- Urinary Pathogen ID/AMR Panel Data Sheet
Support Documentation
Specifications
- Area of interest Microbiology
- Assay time < 9 hr library prep and enrichment time
- Automation capability Liquid handling robot(s)
- Content specifications Detects 120+ bacteria, 14 fungi, 35 viruses, 4 parasites, including 27 sexually transmitted pathogens, and > 3700 antimicrobial resistance markers
- Hands on time < 2 hr library prep and enrichment time
- Input quantity Based on volume (not concentration dependent)
- Instruments MiSeq SystemMiSeq i100 Plus SystemMiSeq i100 SystemMiSeqDx in Research ModeMiniSeq SystemNextSeq 1000 SystemNextSeq 2000 SystemNextSeq 550 SystemNextSeq 550Dx in Research Mode
- Key use Metagenomics, Targeted analysis, Whole-genome analysis
- Mechanism of action On-bead tagmentation followed by a single hybridization step
- Method Target enrichment, Targeted DNA sequencing
- Multiplexing Up to 384 samples in a single run with unique dual indexes
- Nucleic acid type DNA
- Species category Bacteria, Human, Other, Virus
- Sub category Library preparation kits
- Technology Sequencing
Components
- 20142569 — UPIP, Urinary Pathogen ID/AMR Panel
- 20026215 — NXTR Lib Prep Buffers - 96 Smpls
- 20026214 — Illumina DNA / RNA Prep Tagmentation Beads (S) 96
- 20026213 — NXTR Lib Prep -PCR Reagents- 96 Smpls
- 20026210 — NXTR Enrich- Bead + Buff - 96 Smpls
- 20041737 — ILMN RNA Single Hyb Enrich PCR 96
- 20091646 — ILMN DNA/RNA UD Index Set A
links and documants
- Datasheet url Click here
- Source url Click here
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Urinary Pathogen ID/AMR Enrichment Kit Set A (RUO) (96 indexes, 96 samples)
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