Uracil N-Glycosylase (UNG), also known as uracil-DNA glycosylase, hydrolyses the N-glycosidic bond between the deoxyribose sugar and uracil in DNA containing deoxyuridine in place of thymidine. UNG is active on both ssDNA and dsDNA that contain uracil, but has no activity on RNA or 2´-deoxyuridine- 5´-monophosphate. UNG is ideal for studying repair of abasic sites in double-stranded DNA.
Key features
Cleave any DNA containing dUTP in place of dTTP
Hydrolyses -the N-glycosidic bond between the deoxyribose sugar and uracil in DNA containing deoxyuridine in place of thymidine
Well suited for use in DNA repair research studies
Specifications
Unit Definition: One unit of UNG catalyses the release of 1 nmol of uracil from uracil-containing DNA in 1 hour at 37 °C under standard assay conditions.
Note: Epicentre's unit is 5- to 20-fold more active than a unit as defined by other suppliers. Therefore, Dilution Buffer is provided for applications requiring lower concentrations of enzyme.
Dilution and Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.
Quality Control: UNG is free of detectable exo- and endonuclease and RNase activities.
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Product Details
Kit contains Uracil N-Glycosylase (UNG) and UNG Storage Dilution Buffer.
