The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.
Features
Easy, plate-based workflow—direct input of single cells isolated by FACS or other methods
Single-tube workflows—complete cDNA synthesis and library preparation, in one tube each, for minimization of sample loss, sample mix-up, or other handling errors
Unparalleled sensitivity and reproducibility—get the best performance for single-cell RNA-seq (especially for cells with very low RNA content and for nuclei) with the highest gene detection
Increased sequencing power—include unique dual indexes to allow for the pooling of multiple samples and confident sequencing on the NovaSeq™ system
High library yield—sequence on high-throughput sequencers with ease (such as the NovaSeq system) or confidently save extra library for future runs
Applications
cDNA synthesis from single cells for full-length transcriptome sequencing cDNA outputs from SMART-Seq mRNA Single Cell can be used with SMART-Seq Library Prep (not sold separately; sold as part of SMART-Seq mRNA Single Cell LP) for Illumina sequencing
our patented library preparation kit that incorporates enzymatic fragmentation and stem-loop adapters to construct high-quality, Illumina-compatible libraries from the cDNA synthesized with the core kit
