The Tribio® Ultra-sensitive Human IL-33 Fast ELISA is a solid phase ELISA designed to measure human IL-33 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and ultra-sensitive. The measurement can detect a concentration as low as 0.5pg/mL, and finish in 2 hours, with no need for 5-6 hours (Fig. 1). The detection range is from 0.5 to 2048 pg/mL. The levels of human IL-33 samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human IL-33 protein.
Simple Procedures
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
| Part |
Part# |
Description |
Storage of Opened/Reconstituted |
| Human IL-33 Microplate |
TBS3245A |
96 well strip microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human IL-33. |
Return unused wells to the foil pouch containing the desiccant pack. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Human IL-33 Standard |
TBS3245B |
100 µL of Recombinant human IL-33 protein (100 ng/mL). |
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A |
TBS3245C |
2.2 ml of HRP-Human IL-33 antibody. |
May be stored for up to 3 months at 2-8 °C. |
| Assay Diluent |
TBS3245D |
12 ml of a buffered protein base with preservatives. |
| Wash Buffer |
TBS3000W |
12 mL of concentrated solution (10x). |
| TMB Substrate |
TBS3000T |
12 mL of ultra-sensitive TMB substrate. |
| Stop Solution |
TBS3000S |
6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
Related Products
- Human IL-1ß ELISA (TBS3219)
- Human IL-2 ELISA (TBS3220)
- Human IL-4 ELISA (TBS3221)
- Human IL-6 ELISA (TBS3223)
- Human IL-7 ELISA (TBS3224)
Research use only.
Documents
References
1. Tan, M., Ye, W., Liu, Y., Chen, X., Huttad, L., Chua, M. S., & So, S. (2024). Niclosamide Prodrug Enhances Oral Bioavailability and Targets Vasorin-TGFβ Signaling in Hepatocellular Carcinoma. biorxiv. https://doi.org/10.1101/2024.10.15.618538
2. Tan, M., Ye, W., Liu, Y., Chen, X., Huttad, L., Chua, M. S., & So, S. (2025). Niclosamide Prodrug Enhances Oral Bioavailability and Efficacy Against Hepatocellular Carcinoma by Targeting Vasorin-TGFβ Signaling. Authorea. https://doi.org/10.22541/au.174119415.55528730/v1
Research use only.
