The Tribio® Fast Human IFN- γ ELISA Maxi is a solid phase ELISA designed to measure human IFN- γ levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 3 hours, no need for 5-6 hours (Fig. 1). The detection range is from 6 to 4860 pg/mL. The levels of human IFN- γ samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IFN- γ protein.
Typical Data
This standard curve is provided for demonstration only as Fig.3. A standard curve should be generated for each set of samples assayed.
Sensitivity
The minimum detectable dose (MOD) of human IFN-γ is typically 6 pg/ml.
Specificity
This assay recognizes natural and recombinant human IFN- γ.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
HumanIFN- γ Microplate
TBS3230UA
96 well strip microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human IFN- γ.
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
HumanIFN- γ Standard
TBS3230UB
50 µL of Recombinant human IFN- γ protein (145.8ng/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS3230UC
2.2 mL of HRP-Human IFN- γ antibody.
May be stored for up to 3 months at 2-8 °C.
Assay Diluent
TBS3230UD
12 mL of a buffered protein base with preservatives.
Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8℃. Do not use past kit expiration date.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
