The Ultra-sensitive Human TGF-β1 ELISA is a solid phase ELISA to measure the low concentration of human TGF-β1 at 0.5pg/mL level in cell culture supernatants, serum, and plasma, which is not possible to be detected with the regular ELISA. The main feature of this kit is the use of a novel approach to replace traditional methods. The kit is designed to ensure short steps, a short incubation time, and only one-step washing out. The measurement can be finished within 2 hours instead of the traditional 4-5 hours. The detection range is between 0.5-2048 pg/mL. The levels of human TGF-β1 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human TGF-β1 protein.
Simple Procedures
Simple Standard Preparation
Accurate Standard Curve
Kit Contents and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Human TGF-β1 Microplate
TBS3232UA
96-well microplate (12-strips of 8-wells) coated with a Capture Antibody specific for human TGF-β1.
The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.
Human TGF-β1 Standard
TBS3232UB
25 µL of Recombinant human TGF-β1protein (102.4 ng/mL).
Aliquot and store at -20 ℃ for up to 6 months in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS3232UC
2.2 mL of human TGF-β1 antibody.
May be stored for up to 3 months at 2-8℃
Detection B
TBS3232UD
12 mL of Streptavidin-HRP (1x)
Assay Diluent
TBS3232UE
12 mL of buffered protein base with preservatives.
10x Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
